TY - JOUR
T1 - A reliable and reproducible technique for DNA fingerprinting in biorepositories
T2 - A pilot study from BioBIM
AU - Palmirotta, Raffaele
AU - De Marchis, Maria Laura
AU - Ludovici, Giorgia
AU - Ialongo, Cristiano
AU - Leone, Barbara
AU - Lopez, Nadia
AU - Valente, Maria Giovanna
AU - Spila, Antonella
AU - Ferroni, Patrizia
AU - Della-Morte, David
AU - Guadagni, Fiorella
PY - 2013/10
Y1 - 2013/10
N2 - Standard operating procedures (SOPs) optimization for nucleic acid extraction from stored samples is of crucial importance in a biological repository, considering the large number of collected samples and their future downstream molecular and biological applications. However, the validity of molecular studies using stored specimens depends not only on the integrity of the biological samples, but also on the procedures that ensure the traceability of the same sample, certifying its uniqueness, and ensuring the identification of potential sample contaminations. With this aim, we have developed a rapid, reliable, low-cost, and simple DNA fingerprinting tool for a routine use in quality control of biorepositories samples. The method consists of a double ALU insertion/deletion genotyping panel suitable for uniqueness, identification of sample contaminations, and gender validation. Preliminary data suggest that this easy-to-use DNA fingerprinting protocol could routinely provide assurances of DNA identity and quality in a biorepository setting.
AB - Standard operating procedures (SOPs) optimization for nucleic acid extraction from stored samples is of crucial importance in a biological repository, considering the large number of collected samples and their future downstream molecular and biological applications. However, the validity of molecular studies using stored specimens depends not only on the integrity of the biological samples, but also on the procedures that ensure the traceability of the same sample, certifying its uniqueness, and ensuring the identification of potential sample contaminations. With this aim, we have developed a rapid, reliable, low-cost, and simple DNA fingerprinting tool for a routine use in quality control of biorepositories samples. The method consists of a double ALU insertion/deletion genotyping panel suitable for uniqueness, identification of sample contaminations, and gender validation. Preliminary data suggest that this easy-to-use DNA fingerprinting protocol could routinely provide assurances of DNA identity and quality in a biorepository setting.
KW - Biobank
KW - Biospecimens
KW - DNA fingerprint
UR - http://www.scopus.com/inward/record.url?scp=84890715063&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84890715063&partnerID=8YFLogxK
U2 - 10.5301/JBM.5000044
DO - 10.5301/JBM.5000044
M3 - Article
C2 - 23873620
AN - SCOPUS:84890715063
VL - 28
JO - International Journal of Biological Markers
JF - International Journal of Biological Markers
SN - 0393-6155
IS - 4
ER -