A review of research-grade human induced pluripotent stem cells qualification and biobanking processes

Aikaterini Ntai, Simona Baronchelli, Alberto La Spada, Anna Moles, Alessandro Guffanti, Pasquale De Blasio, Ida Biunno

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Human induced pluripotent stem cell (hiPSC) biobanks are invaluable resources for basic and clinical research, since they provide a sustainable supply of accessible cell lines that meet high quality and safety standards. hiPSCs are particularly useful for understanding disease mechanisms, creating cell models for drug development, and generating novel clinical therapies. For clinical applications and drug discovery, it is fundamental that the acquired pluripotent cell lines never touch animal-derived products nor xenogeneic reagents (Good Manufacturing Practice - grade); whereas for research grade, it is sufficient to operate under Good Laboratory Practice conditions. However, regardless of the end use, it is important that every step in the whole process, starting from the original cells throughout expansion and manipulation, must be performed and recorded rigorously. Here, we describe our biobanking management system that is applied specifically to human pluripotent stem cells.

Original languageEnglish
Pages (from-to)384-392
Number of pages9
JournalBiopreservation and Biobanking
Volume15
Issue number4
DOIs
Publication statusPublished - Aug 1 2017

Fingerprint

Induced Pluripotent Stem Cells
Stem cells
Cells
Cell Line
Pluripotent Stem Cells
Touch
Drug Discovery
Research
Animals
Safety
Pharmaceutical Preparations
Therapeutics

Keywords

  • cell banking
  • human induced pluripotent stem cells
  • quality control

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology

Cite this

Ntai, A., Baronchelli, S., La Spada, A., Moles, A., Guffanti, A., De Blasio, P., & Biunno, I. (2017). A review of research-grade human induced pluripotent stem cells qualification and biobanking processes. Biopreservation and Biobanking, 15(4), 384-392. https://doi.org/10.1089/bio.2016.0097

A review of research-grade human induced pluripotent stem cells qualification and biobanking processes. / Ntai, Aikaterini; Baronchelli, Simona; La Spada, Alberto; Moles, Anna; Guffanti, Alessandro; De Blasio, Pasquale; Biunno, Ida.

In: Biopreservation and Biobanking, Vol. 15, No. 4, 01.08.2017, p. 384-392.

Research output: Contribution to journalArticle

Ntai, A, Baronchelli, S, La Spada, A, Moles, A, Guffanti, A, De Blasio, P & Biunno, I 2017, 'A review of research-grade human induced pluripotent stem cells qualification and biobanking processes', Biopreservation and Biobanking, vol. 15, no. 4, pp. 384-392. https://doi.org/10.1089/bio.2016.0097
Ntai, Aikaterini ; Baronchelli, Simona ; La Spada, Alberto ; Moles, Anna ; Guffanti, Alessandro ; De Blasio, Pasquale ; Biunno, Ida. / A review of research-grade human induced pluripotent stem cells qualification and biobanking processes. In: Biopreservation and Biobanking. 2017 ; Vol. 15, No. 4. pp. 384-392.
@article{fcff77f4b9a34bc5835d81b404c3bfa2,
title = "A review of research-grade human induced pluripotent stem cells qualification and biobanking processes",
abstract = "Human induced pluripotent stem cell (hiPSC) biobanks are invaluable resources for basic and clinical research, since they provide a sustainable supply of accessible cell lines that meet high quality and safety standards. hiPSCs are particularly useful for understanding disease mechanisms, creating cell models for drug development, and generating novel clinical therapies. For clinical applications and drug discovery, it is fundamental that the acquired pluripotent cell lines never touch animal-derived products nor xenogeneic reagents (Good Manufacturing Practice - grade); whereas for research grade, it is sufficient to operate under Good Laboratory Practice conditions. However, regardless of the end use, it is important that every step in the whole process, starting from the original cells throughout expansion and manipulation, must be performed and recorded rigorously. Here, we describe our biobanking management system that is applied specifically to human pluripotent stem cells.",
keywords = "cell banking, human induced pluripotent stem cells, quality control",
author = "Aikaterini Ntai and Simona Baronchelli and {La Spada}, Alberto and Anna Moles and Alessandro Guffanti and {De Blasio}, Pasquale and Ida Biunno",
year = "2017",
month = "8",
day = "1",
doi = "10.1089/bio.2016.0097",
language = "English",
volume = "15",
pages = "384--392",
journal = "Biopreservation and Biobanking",
issn = "1947-5535",
publisher = "Mary Ann Liebert Inc.",
number = "4",

}

TY - JOUR

T1 - A review of research-grade human induced pluripotent stem cells qualification and biobanking processes

AU - Ntai, Aikaterini

AU - Baronchelli, Simona

AU - La Spada, Alberto

AU - Moles, Anna

AU - Guffanti, Alessandro

AU - De Blasio, Pasquale

AU - Biunno, Ida

PY - 2017/8/1

Y1 - 2017/8/1

N2 - Human induced pluripotent stem cell (hiPSC) biobanks are invaluable resources for basic and clinical research, since they provide a sustainable supply of accessible cell lines that meet high quality and safety standards. hiPSCs are particularly useful for understanding disease mechanisms, creating cell models for drug development, and generating novel clinical therapies. For clinical applications and drug discovery, it is fundamental that the acquired pluripotent cell lines never touch animal-derived products nor xenogeneic reagents (Good Manufacturing Practice - grade); whereas for research grade, it is sufficient to operate under Good Laboratory Practice conditions. However, regardless of the end use, it is important that every step in the whole process, starting from the original cells throughout expansion and manipulation, must be performed and recorded rigorously. Here, we describe our biobanking management system that is applied specifically to human pluripotent stem cells.

AB - Human induced pluripotent stem cell (hiPSC) biobanks are invaluable resources for basic and clinical research, since they provide a sustainable supply of accessible cell lines that meet high quality and safety standards. hiPSCs are particularly useful for understanding disease mechanisms, creating cell models for drug development, and generating novel clinical therapies. For clinical applications and drug discovery, it is fundamental that the acquired pluripotent cell lines never touch animal-derived products nor xenogeneic reagents (Good Manufacturing Practice - grade); whereas for research grade, it is sufficient to operate under Good Laboratory Practice conditions. However, regardless of the end use, it is important that every step in the whole process, starting from the original cells throughout expansion and manipulation, must be performed and recorded rigorously. Here, we describe our biobanking management system that is applied specifically to human pluripotent stem cells.

KW - cell banking

KW - human induced pluripotent stem cells

KW - quality control

UR - http://www.scopus.com/inward/record.url?scp=85027236671&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85027236671&partnerID=8YFLogxK

U2 - 10.1089/bio.2016.0097

DO - 10.1089/bio.2016.0097

M3 - Article

C2 - 28388226

AN - SCOPUS:85027236671

VL - 15

SP - 384

EP - 392

JO - Biopreservation and Biobanking

JF - Biopreservation and Biobanking

SN - 1947-5535

IS - 4

ER -