A review of research-grade human induced pluripotent stem cells qualification and biobanking processes

Aikaterini Ntai, Simona Baronchelli, Alberto La Spada, Anna Moles, Alessandro Guffanti, Pasquale De Blasio, Ida Biunno

Research output: Contribution to journalArticlepeer-review

Abstract

Human induced pluripotent stem cell (hiPSC) biobanks are invaluable resources for basic and clinical research, since they provide a sustainable supply of accessible cell lines that meet high quality and safety standards. hiPSCs are particularly useful for understanding disease mechanisms, creating cell models for drug development, and generating novel clinical therapies. For clinical applications and drug discovery, it is fundamental that the acquired pluripotent cell lines never touch animal-derived products nor xenogeneic reagents (Good Manufacturing Practice - grade); whereas for research grade, it is sufficient to operate under Good Laboratory Practice conditions. However, regardless of the end use, it is important that every step in the whole process, starting from the original cells throughout expansion and manipulation, must be performed and recorded rigorously. Here, we describe our biobanking management system that is applied specifically to human pluripotent stem cells.

Original languageEnglish
Pages (from-to)384-392
Number of pages9
JournalBiopreservation and Biobanking
Volume15
Issue number4
DOIs
Publication statusPublished - Aug 1 2017

Keywords

  • cell banking
  • human induced pluripotent stem cells
  • quality control

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology

Fingerprint Dive into the research topics of 'A review of research-grade human induced pluripotent stem cells qualification and biobanking processes'. Together they form a unique fingerprint.

Cite this