A role for phosphatidic acid in COPI vesicle fission yields insights into Golgi maintenance

Jia Shu Yang, Helge Gad, Stella Y. Lee, Alexander Mironov, Leiliang Zhang, Galina V. Beznoussenko, Carmen Valente, Gabriele Turacchio, Akua N. Bonsra, Guangwei Du, Gianluca Baldanzi, Andrea Graziani, Sylvain Bourgoin, Michael A. Frohman, Alberto Luini, Victor W. Hsu

Research output: Contribution to journalArticlepeer-review


Proteins essential for vesicle formation by the Coat Protein I (COPI) complex are being identified, but less is known about the role of specific lipids. Brefeldin-A ADP-ribosylated substrate (BARS) functions in the fission step of COPI vesicle formation. Here, we show that BARS induces membrane curvature in cooperation with phosphatidic acid. This finding has allowed us to further delineate COPI vesicle fission into two sub-stages: 1) an earlier stage of bud-neck constriction, in which BARS and other COPI components are required, and 2) a later stage of bud-neck scission, in which phosphatidic acid generated by phospholipase D2 (PLD2) is also required. Moreover, in contrast to the disruption of the Golgi seen on perturbing the core COPI components (such as coatomer), inhibition of PLD2 causes milder disruptions, suggesting that such COPI components have additional roles in maintaining Golgi structure other than through COPI vesicle formation.

Original languageEnglish
Pages (from-to)1146-1153
Number of pages8
JournalNature Cell Biology
Issue number10
Publication statusPublished - 2008

ASJC Scopus subject areas

  • Cell Biology


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