A role for phosphatidic acid in COPI vesicle fission yields insights into Golgi maintenance

Jia Shu Yang, Helge Gad, Stella Y. Lee, Alexander Mironov, Leiliang Zhang, Galina V. Beznoussenko, Carmen Valente, Gabriele Turacchio, Akua N. Bonsra, Guangwei Du, Gianluca Baldanzi, Andrea Graziani, Sylvain Bourgoin, Michael A. Frohman, Alberto Luini, Victor W. Hsu

Research output: Contribution to journalArticle

117 Citations (Scopus)

Abstract

Proteins essential for vesicle formation by the Coat Protein I (COPI) complex are being identified, but less is known about the role of specific lipids. Brefeldin-A ADP-ribosylated substrate (BARS) functions in the fission step of COPI vesicle formation. Here, we show that BARS induces membrane curvature in cooperation with phosphatidic acid. This finding has allowed us to further delineate COPI vesicle fission into two sub-stages: 1) an earlier stage of bud-neck constriction, in which BARS and other COPI components are required, and 2) a later stage of bud-neck scission, in which phosphatidic acid generated by phospholipase D2 (PLD2) is also required. Moreover, in contrast to the disruption of the Golgi seen on perturbing the core COPI components (such as coatomer), inhibition of PLD2 causes milder disruptions, suggesting that such COPI components have additional roles in maintaining Golgi structure other than through COPI vesicle formation.

Original languageEnglish
Pages (from-to)1146-1153
Number of pages8
JournalNature Cell Biology
Volume10
Issue number10
DOIs
Publication statusPublished - 2008

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Phosphatidic Acids
Capsid Proteins
Maintenance
Brefeldin A
Adenosine Diphosphate
Neck
Coat Protein Complex I
Constriction
Lipids
Membranes
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Yang, J. S., Gad, H., Lee, S. Y., Mironov, A., Zhang, L., Beznoussenko, G. V., ... Hsu, V. W. (2008). A role for phosphatidic acid in COPI vesicle fission yields insights into Golgi maintenance. Nature Cell Biology, 10(10), 1146-1153. https://doi.org/10.1038/ncb1774

A role for phosphatidic acid in COPI vesicle fission yields insights into Golgi maintenance. / Yang, Jia Shu; Gad, Helge; Lee, Stella Y.; Mironov, Alexander; Zhang, Leiliang; Beznoussenko, Galina V.; Valente, Carmen; Turacchio, Gabriele; Bonsra, Akua N.; Du, Guangwei; Baldanzi, Gianluca; Graziani, Andrea; Bourgoin, Sylvain; Frohman, Michael A.; Luini, Alberto; Hsu, Victor W.

In: Nature Cell Biology, Vol. 10, No. 10, 2008, p. 1146-1153.

Research output: Contribution to journalArticle

Yang, JS, Gad, H, Lee, SY, Mironov, A, Zhang, L, Beznoussenko, GV, Valente, C, Turacchio, G, Bonsra, AN, Du, G, Baldanzi, G, Graziani, A, Bourgoin, S, Frohman, MA, Luini, A & Hsu, VW 2008, 'A role for phosphatidic acid in COPI vesicle fission yields insights into Golgi maintenance', Nature Cell Biology, vol. 10, no. 10, pp. 1146-1153. https://doi.org/10.1038/ncb1774
Yang JS, Gad H, Lee SY, Mironov A, Zhang L, Beznoussenko GV et al. A role for phosphatidic acid in COPI vesicle fission yields insights into Golgi maintenance. Nature Cell Biology. 2008;10(10):1146-1153. https://doi.org/10.1038/ncb1774
Yang, Jia Shu ; Gad, Helge ; Lee, Stella Y. ; Mironov, Alexander ; Zhang, Leiliang ; Beznoussenko, Galina V. ; Valente, Carmen ; Turacchio, Gabriele ; Bonsra, Akua N. ; Du, Guangwei ; Baldanzi, Gianluca ; Graziani, Andrea ; Bourgoin, Sylvain ; Frohman, Michael A. ; Luini, Alberto ; Hsu, Victor W. / A role for phosphatidic acid in COPI vesicle fission yields insights into Golgi maintenance. In: Nature Cell Biology. 2008 ; Vol. 10, No. 10. pp. 1146-1153.
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