A scanning electron microscopy analysis of human cytotoxic cell subsets and of their mode of conjugation with tumor cell targets

W. Malorni, G. Arancia, F. Iosi, G. Donelli, D. Zarcone, A. Moretta, C. E. Grossi

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In this study we describe the surface features of non-MHC (Major Histocompatibility Complex)-restricted cytotoxic cells isolated from human peripheral blood. Purified populations of CD3-, natural killer (NK) cells were allowed to interact with NK-sensitive (K562) and NK-resistant (THP-1-0) tumor cell targets. The type of effector to target cell binding was investigated by scanning electron microscope (SEM) analysis. A different interaction with the effectors is described for NK-resistant targets in comparison with NK-susceptible tumor cells. SEM was also used to investigate the relationship between interleukin 2 (IL2)-activated cytotoxic cells (lymphokine-activated killer, LAK, cells) and the tumor targets. We also describe the unique growth features of certain clones of cytotoxic T cells expressing γδ antigen receptors which support the contention that these cells may have a special ability of homing into tissues. We conclude that non-specific cytotoxic cells constitute a diverse population of effectors which differ not only for the expression of surface antigens, but also for their ability to interact with tumor cell targets and to home into the peripheral tissues where they may exert their lytic functions.

Original languageEnglish
Pages (from-to)919-927
Number of pages9
JournalScanning Microscopy
Volume3
Issue number3
Publication statusPublished - 1989

Fingerprint

conjugation
set theory
Tumors
tumors
Cells
Scanning electron microscopy
scanning electron microscopy
effectors
Antigens
cells
Electron microscopes
Tissue
Scanning
antigens
T-cells
electron microscopes
homing
Blood
interleukins
scanning

ASJC Scopus subject areas

  • Instrumentation

Cite this

Malorni, W., Arancia, G., Iosi, F., Donelli, G., Zarcone, D., Moretta, A., & Grossi, C. E. (1989). A scanning electron microscopy analysis of human cytotoxic cell subsets and of their mode of conjugation with tumor cell targets. Scanning Microscopy, 3(3), 919-927.

A scanning electron microscopy analysis of human cytotoxic cell subsets and of their mode of conjugation with tumor cell targets. / Malorni, W.; Arancia, G.; Iosi, F.; Donelli, G.; Zarcone, D.; Moretta, A.; Grossi, C. E.

In: Scanning Microscopy, Vol. 3, No. 3, 1989, p. 919-927.

Research output: Contribution to journalArticle

Malorni, W, Arancia, G, Iosi, F, Donelli, G, Zarcone, D, Moretta, A & Grossi, CE 1989, 'A scanning electron microscopy analysis of human cytotoxic cell subsets and of their mode of conjugation with tumor cell targets', Scanning Microscopy, vol. 3, no. 3, pp. 919-927.
Malorni, W. ; Arancia, G. ; Iosi, F. ; Donelli, G. ; Zarcone, D. ; Moretta, A. ; Grossi, C. E. / A scanning electron microscopy analysis of human cytotoxic cell subsets and of their mode of conjugation with tumor cell targets. In: Scanning Microscopy. 1989 ; Vol. 3, No. 3. pp. 919-927.
@article{417db44388b24097839980ebceec0cc3,
title = "A scanning electron microscopy analysis of human cytotoxic cell subsets and of their mode of conjugation with tumor cell targets",
abstract = "In this study we describe the surface features of non-MHC (Major Histocompatibility Complex)-restricted cytotoxic cells isolated from human peripheral blood. Purified populations of CD3-, natural killer (NK) cells were allowed to interact with NK-sensitive (K562) and NK-resistant (THP-1-0) tumor cell targets. The type of effector to target cell binding was investigated by scanning electron microscope (SEM) analysis. A different interaction with the effectors is described for NK-resistant targets in comparison with NK-susceptible tumor cells. SEM was also used to investigate the relationship between interleukin 2 (IL2)-activated cytotoxic cells (lymphokine-activated killer, LAK, cells) and the tumor targets. We also describe the unique growth features of certain clones of cytotoxic T cells expressing γδ antigen receptors which support the contention that these cells may have a special ability of homing into tissues. We conclude that non-specific cytotoxic cells constitute a diverse population of effectors which differ not only for the expression of surface antigens, but also for their ability to interact with tumor cell targets and to home into the peripheral tissues where they may exert their lytic functions.",
author = "W. Malorni and G. Arancia and F. Iosi and G. Donelli and D. Zarcone and A. Moretta and Grossi, {C. E.}",
year = "1989",
language = "English",
volume = "3",
pages = "919--927",
journal = "Scanning Microscopy",
issn = "0891-7035",
publisher = "Scanning Microscopy International",
number = "3",

}

TY - JOUR

T1 - A scanning electron microscopy analysis of human cytotoxic cell subsets and of their mode of conjugation with tumor cell targets

AU - Malorni, W.

AU - Arancia, G.

AU - Iosi, F.

AU - Donelli, G.

AU - Zarcone, D.

AU - Moretta, A.

AU - Grossi, C. E.

PY - 1989

Y1 - 1989

N2 - In this study we describe the surface features of non-MHC (Major Histocompatibility Complex)-restricted cytotoxic cells isolated from human peripheral blood. Purified populations of CD3-, natural killer (NK) cells were allowed to interact with NK-sensitive (K562) and NK-resistant (THP-1-0) tumor cell targets. The type of effector to target cell binding was investigated by scanning electron microscope (SEM) analysis. A different interaction with the effectors is described for NK-resistant targets in comparison with NK-susceptible tumor cells. SEM was also used to investigate the relationship between interleukin 2 (IL2)-activated cytotoxic cells (lymphokine-activated killer, LAK, cells) and the tumor targets. We also describe the unique growth features of certain clones of cytotoxic T cells expressing γδ antigen receptors which support the contention that these cells may have a special ability of homing into tissues. We conclude that non-specific cytotoxic cells constitute a diverse population of effectors which differ not only for the expression of surface antigens, but also for their ability to interact with tumor cell targets and to home into the peripheral tissues where they may exert their lytic functions.

AB - In this study we describe the surface features of non-MHC (Major Histocompatibility Complex)-restricted cytotoxic cells isolated from human peripheral blood. Purified populations of CD3-, natural killer (NK) cells were allowed to interact with NK-sensitive (K562) and NK-resistant (THP-1-0) tumor cell targets. The type of effector to target cell binding was investigated by scanning electron microscope (SEM) analysis. A different interaction with the effectors is described for NK-resistant targets in comparison with NK-susceptible tumor cells. SEM was also used to investigate the relationship between interleukin 2 (IL2)-activated cytotoxic cells (lymphokine-activated killer, LAK, cells) and the tumor targets. We also describe the unique growth features of certain clones of cytotoxic T cells expressing γδ antigen receptors which support the contention that these cells may have a special ability of homing into tissues. We conclude that non-specific cytotoxic cells constitute a diverse population of effectors which differ not only for the expression of surface antigens, but also for their ability to interact with tumor cell targets and to home into the peripheral tissues where they may exert their lytic functions.

UR - http://www.scopus.com/inward/record.url?scp=0024814164&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024814164&partnerID=8YFLogxK

M3 - Article

C2 - 2617272

AN - SCOPUS:0024814164

VL - 3

SP - 919

EP - 927

JO - Scanning Microscopy

JF - Scanning Microscopy

SN - 0891-7035

IS - 3

ER -