A screen for RAS mutations in individuals at risk of secondary leukaemia due to occupational exposure to petrochemicals

Christine Taylor, David C. Hughes, Elisabetta Zappone, Mario Cazzola, Graham Carter, Allan Jacobs, Rose Ann Padua

Research output: Contribution to journalArticle

Abstract

Occupational exposure to petrochemicals, in particular benzene, has been identified as a risk factor in the development of acute leukaemia. A cohort of exposed (n = 44) and nonexposed individuals (n = 19) from the same petrochemical installation were screened by polymerase chain reaction (PCR) followed by oligonucleotide hybridization (ONH) for the presence of mutations in the H, K, and NRAS cellular proto-oncogenes. A KRAS mutation was detected in one individual from the exposed group who was haematologically normal at the time of sampling. The presence of this mutation was confirmed by nude mouse tumorigenicity assay and positively identified as a K13 Gly-Asp substitution by cloning and sequencing.

Original languageEnglish
Pages (from-to)299-301
Number of pages3
JournalLeukemia Research
Volume19
Issue number5
DOIs
Publication statusPublished - 1995

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Keywords

  • petrochemical exposure
  • RAS proto-oncogenes
  • secondary leukaemia

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

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