Abstract
Occupational exposure to petrochemicals, in particular benzene, has been identified as a risk factor in the development of acute leukaemia. A cohort of exposed (n = 44) and nonexposed individuals (n = 19) from the same petrochemical installation were screened by polymerase chain reaction (PCR) followed by oligonucleotide hybridization (ONH) for the presence of mutations in the H, K, and NRAS cellular proto-oncogenes. A KRAS mutation was detected in one individual from the exposed group who was haematologically normal at the time of sampling. The presence of this mutation was confirmed by nude mouse tumorigenicity assay and positively identified as a K13 Gly-Asp substitution by cloning and sequencing.
Original language | English |
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Pages (from-to) | 299-301 |
Number of pages | 3 |
Journal | Leukemia Research |
Volume | 19 |
Issue number | 5 |
DOIs | |
Publication status | Published - 1995 |
Keywords
- petrochemical exposure
- RAS proto-oncogenes
- secondary leukaemia
ASJC Scopus subject areas
- Cancer Research
- Hematology
- Oncology