A screen for RAS mutations in individuals at risk of secondary leukaemia due to occupational exposure to petrochemicals

Christine Taylor; David C. Hughes; Elisabetta Zappone; Mario Cazzola; Graham Carter; Allan Jacobs; Rose Ann Padua

doi:10.1016/0145-2126(95)00005-9

A screen for RAS mutations in individuals at risk of secondary leukaemia due to occupational exposure to petrochemicals

Christine Taylor, David C. Hughes, Elisabetta Zappone, Mario Cazzola, Graham Carter, Allan Jacobs, Rose Ann Padua

IRCCS Fondazione Policlinico San Matteo

Research output: Contribution to journal › Article › peer-review

Abstract

Occupational exposure to petrochemicals, in particular benzene, has been identified as a risk factor in the development of acute leukaemia. A cohort of exposed (n = 44) and nonexposed individuals (n = 19) from the same petrochemical installation were screened by polymerase chain reaction (PCR) followed by oligonucleotide hybridization (ONH) for the presence of mutations in the H, K, and NRAS cellular proto-oncogenes. A KRAS mutation was detected in one individual from the exposed group who was haematologically normal at the time of sampling. The presence of this mutation was confirmed by nude mouse tumorigenicity assay and positively identified as a K13 Gly-Asp substitution by cloning and sequencing.

Original language	English
Pages (from-to)	299-301
Number of pages	3
Journal	Leukemia Research
Volume	19
Issue number	5
DOIs	https://doi.org/10.1016/0145-2126(95)00005-9
Publication status	Published - 1995

Keywords

petrochemical exposure
RAS proto-oncogenes
secondary leukaemia

ASJC Scopus subject areas

Cancer Research
Hematology
Oncology

Access to Document

10.1016/0145-2126(95)00005-9

Cite this

@article{5e0669aff5364b9fa47e3f7aa2786f8a,

title = "A screen for RAS mutations in individuals at risk of secondary leukaemia due to occupational exposure to petrochemicals",

abstract = "Occupational exposure to petrochemicals, in particular benzene, has been identified as a risk factor in the development of acute leukaemia. A cohort of exposed (n = 44) and nonexposed individuals (n = 19) from the same petrochemical installation were screened by polymerase chain reaction (PCR) followed by oligonucleotide hybridization (ONH) for the presence of mutations in the H, K, and NRAS cellular proto-oncogenes. A KRAS mutation was detected in one individual from the exposed group who was haematologically normal at the time of sampling. The presence of this mutation was confirmed by nude mouse tumorigenicity assay and positively identified as a K13 Gly-Asp substitution by cloning and sequencing.",

keywords = "petrochemical exposure, RAS proto-oncogenes, secondary leukaemia",

author = "Christine Taylor and Hughes, {David C.} and Elisabetta Zappone and Mario Cazzola and Graham Carter and Allan Jacobs and Padua, {Rose Ann}",

year = "1995",

doi = "10.1016/0145-2126(95)00005-9",

language = "English",

volume = "19",

pages = "299--301",

journal = "Leukemia Research",

issn = "0145-2126",

publisher = "Elsevier Ltd",

number = "5",

}

TY - JOUR

T1 - A screen for RAS mutations in individuals at risk of secondary leukaemia due to occupational exposure to petrochemicals

AU - Taylor, Christine

AU - Hughes, David C.

AU - Zappone, Elisabetta

AU - Cazzola, Mario

AU - Carter, Graham

AU - Jacobs, Allan

AU - Padua, Rose Ann

PY - 1995

Y1 - 1995

N2 - Occupational exposure to petrochemicals, in particular benzene, has been identified as a risk factor in the development of acute leukaemia. A cohort of exposed (n = 44) and nonexposed individuals (n = 19) from the same petrochemical installation were screened by polymerase chain reaction (PCR) followed by oligonucleotide hybridization (ONH) for the presence of mutations in the H, K, and NRAS cellular proto-oncogenes. A KRAS mutation was detected in one individual from the exposed group who was haematologically normal at the time of sampling. The presence of this mutation was confirmed by nude mouse tumorigenicity assay and positively identified as a K13 Gly-Asp substitution by cloning and sequencing.

AB - Occupational exposure to petrochemicals, in particular benzene, has been identified as a risk factor in the development of acute leukaemia. A cohort of exposed (n = 44) and nonexposed individuals (n = 19) from the same petrochemical installation were screened by polymerase chain reaction (PCR) followed by oligonucleotide hybridization (ONH) for the presence of mutations in the H, K, and NRAS cellular proto-oncogenes. A KRAS mutation was detected in one individual from the exposed group who was haematologically normal at the time of sampling. The presence of this mutation was confirmed by nude mouse tumorigenicity assay and positively identified as a K13 Gly-Asp substitution by cloning and sequencing.

KW - petrochemical exposure

KW - RAS proto-oncogenes

KW - secondary leukaemia

UR - http://www.scopus.com/inward/record.url?scp=0028988863&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028988863&partnerID=8YFLogxK

U2 - 10.1016/0145-2126(95)00005-9

DO - 10.1016/0145-2126(95)00005-9

M3 - Article

C2 - 7769829

AN - SCOPUS:0028988863

VL - 19

SP - 299

EP - 301

JO - Leukemia Research

JF - Leukemia Research

SN - 0145-2126

IS - 5

ER -

A screen for RAS mutations in individuals at risk of secondary leukaemia due to occupational exposure to petrochemicals

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this