Abstract
Dynamin 1 is a neuron-specific guanosine triphosphatase thought to be critically required for the fission reaction of synaptic vesicle endocytosis. Unexpectedly, mice lacking dynamin 1 were able to form functional synapses, even though their postnatal viability was limited. However, during spontaneous network activity, branched, tubular plasma membrane invaginations accumulated, capped by clathrin-coated pits, in synapses of dynamin 1-knockout mice. Synaptic vesicle endocytosis was severely impaired during strong exogenous stimulation but resumed efficiently when the stimulus was terminated. Thus, dynamin 1-independent mechanisms can support limited synaptic vesicle endocytosis, but dynamin 1 is needed during high levels of neuronal activity.
| Original language | English |
|---|---|
| Pages (from-to) | 570-574 |
| Number of pages | 5 |
| Journal | Science |
| Volume | 316 |
| Issue number | 5824 |
| DOIs | |
| Publication status | Published - Apr 27 2007 |
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A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis. / Ferguson, Shawn M.; Brasnjo, Gabor; Hayashi, Mitsuko; Wölfel, Markus; Collesi, Chiara; Giovedi, Silvia; Raimondi, Andrea; Gong, Liang Wei; Ariel, Pablo; Paradise, Summer; O'Toole, Eileen; Flavell, Richard; Cremona, Ottavio; Miesenböck, Gero; Ryan, Timothy A.; De Camilli, Pietro.
In: Science, Vol. 316, No. 5824, 27.04.2007, p. 570-574.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis
AU - Ferguson, Shawn M.
AU - Brasnjo, Gabor
AU - Hayashi, Mitsuko
AU - Wölfel, Markus
AU - Collesi, Chiara
AU - Giovedi, Silvia
AU - Raimondi, Andrea
AU - Gong, Liang Wei
AU - Ariel, Pablo
AU - Paradise, Summer
AU - O'Toole, Eileen
AU - Flavell, Richard
AU - Cremona, Ottavio
AU - Miesenböck, Gero
AU - Ryan, Timothy A.
AU - De Camilli, Pietro
PY - 2007/4/27
Y1 - 2007/4/27
N2 - Dynamin 1 is a neuron-specific guanosine triphosphatase thought to be critically required for the fission reaction of synaptic vesicle endocytosis. Unexpectedly, mice lacking dynamin 1 were able to form functional synapses, even though their postnatal viability was limited. However, during spontaneous network activity, branched, tubular plasma membrane invaginations accumulated, capped by clathrin-coated pits, in synapses of dynamin 1-knockout mice. Synaptic vesicle endocytosis was severely impaired during strong exogenous stimulation but resumed efficiently when the stimulus was terminated. Thus, dynamin 1-independent mechanisms can support limited synaptic vesicle endocytosis, but dynamin 1 is needed during high levels of neuronal activity.
AB - Dynamin 1 is a neuron-specific guanosine triphosphatase thought to be critically required for the fission reaction of synaptic vesicle endocytosis. Unexpectedly, mice lacking dynamin 1 were able to form functional synapses, even though their postnatal viability was limited. However, during spontaneous network activity, branched, tubular plasma membrane invaginations accumulated, capped by clathrin-coated pits, in synapses of dynamin 1-knockout mice. Synaptic vesicle endocytosis was severely impaired during strong exogenous stimulation but resumed efficiently when the stimulus was terminated. Thus, dynamin 1-independent mechanisms can support limited synaptic vesicle endocytosis, but dynamin 1 is needed during high levels of neuronal activity.
UR - http://www.scopus.com/inward/record.url?scp=34247583278&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34247583278&partnerID=8YFLogxK
U2 - 10.1126/science.1140621
DO - 10.1126/science.1140621
M3 - Article
C2 - 17463283
AN - SCOPUS:34247583278
VL - 316
SP - 570
EP - 574
JO - Science
JF - Science
SN - 0036-8075
IS - 5824
ER -