A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis

Shawn M. Ferguson; Gabor Brasnjo; Mitsuko Hayashi; Markus Wölfel; Chiara Collesi; Silvia Giovedi; Andrea Raimondi; Liang Wei Gong; Pablo Ariel; Summer Paradise; Eileen O'Toole; Richard Flavell; Ottavio Cremona; Gero Miesenböck; Timothy A. Ryan; Pietro De Camilli

doi:10.1126/science.1140621

A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis

Shawn M. Ferguson, Gabor Brasnjo, Mitsuko Hayashi, Markus Wölfel, Chiara Collesi, Silvia Giovedi, Andrea Raimondi, Liang Wei Gong, Pablo Ariel, Summer Paradise, Eileen O'Toole, Richard Flavell, Ottavio Cremona, Gero Miesenböck, Timothy A. Ryan, Pietro De Camilli

IRCCS Ospedale San Raffaele

Research output: Contribution to journal › Article › peer-review

Abstract

Dynamin 1 is a neuron-specific guanosine triphosphatase thought to be critically required for the fission reaction of synaptic vesicle endocytosis. Unexpectedly, mice lacking dynamin 1 were able to form functional synapses, even though their postnatal viability was limited. However, during spontaneous network activity, branched, tubular plasma membrane invaginations accumulated, capped by clathrin-coated pits, in synapses of dynamin 1-knockout mice. Synaptic vesicle endocytosis was severely impaired during strong exogenous stimulation but resumed efficiently when the stimulus was terminated. Thus, dynamin 1-independent mechanisms can support limited synaptic vesicle endocytosis, but dynamin 1 is needed during high levels of neuronal activity.

Original language	English
Pages (from-to)	570-574
Number of pages	5
Journal	Science
Volume	316
Issue number	5824
DOIs	https://doi.org/10.1126/science.1140621
Publication status	Published - Apr 27 2007

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Ferguson, S. M., Brasnjo, G., Hayashi, M., Wölfel, M., Collesi, C., Giovedi, S., Raimondi, A., Gong, L. W., Ariel, P., Paradise, S., O'Toole, E., Flavell, R., Cremona, O., Miesenböck, G., Ryan, T. A., & De Camilli, P. (2007). A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis. Science, 316(5824), 570-574. https://doi.org/10.1126/science.1140621

A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis. / Ferguson, Shawn M.; Brasnjo, Gabor; Hayashi, Mitsuko; Wölfel, Markus; Collesi, Chiara; Giovedi, Silvia; Raimondi, Andrea; Gong, Liang Wei; Ariel, Pablo; Paradise, Summer; O'Toole, Eileen; Flavell, Richard; Cremona, Ottavio; Miesenböck, Gero; Ryan, Timothy A.; De Camilli, Pietro.

In: Science, Vol. 316, No. 5824, 27.04.2007, p. 570-574.

Research output: Contribution to journal › Article › peer-review

Ferguson, SM, Brasnjo, G, Hayashi, M, Wölfel, M, Collesi, C, Giovedi, S, Raimondi, A, Gong, LW, Ariel, P, Paradise, S, O'Toole, E, Flavell, R, Cremona, O, Miesenböck, G, Ryan, TA & De Camilli, P 2007, 'A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis', Science, vol. 316, no. 5824, pp. 570-574. https://doi.org/10.1126/science.1140621

Ferguson, Shawn M. ; Brasnjo, Gabor ; Hayashi, Mitsuko ; Wölfel, Markus ; Collesi, Chiara ; Giovedi, Silvia ; Raimondi, Andrea ; Gong, Liang Wei ; Ariel, Pablo ; Paradise, Summer ; O'Toole, Eileen ; Flavell, Richard ; Cremona, Ottavio ; Miesenböck, Gero ; Ryan, Timothy A. ; De Camilli, Pietro. / A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis. In: Science. 2007 ; Vol. 316, No. 5824. pp. 570-574.

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abstract = "Dynamin 1 is a neuron-specific guanosine triphosphatase thought to be critically required for the fission reaction of synaptic vesicle endocytosis. Unexpectedly, mice lacking dynamin 1 were able to form functional synapses, even though their postnatal viability was limited. However, during spontaneous network activity, branched, tubular plasma membrane invaginations accumulated, capped by clathrin-coated pits, in synapses of dynamin 1-knockout mice. Synaptic vesicle endocytosis was severely impaired during strong exogenous stimulation but resumed efficiently when the stimulus was terminated. Thus, dynamin 1-independent mechanisms can support limited synaptic vesicle endocytosis, but dynamin 1 is needed during high levels of neuronal activity.",

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AU - Collesi, Chiara

AU - Giovedi, Silvia

AU - Raimondi, Andrea

AU - Gong, Liang Wei

AU - Ariel, Pablo

AU - Paradise, Summer

AU - O'Toole, Eileen

AU - Flavell, Richard

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AB - Dynamin 1 is a neuron-specific guanosine triphosphatase thought to be critically required for the fission reaction of synaptic vesicle endocytosis. Unexpectedly, mice lacking dynamin 1 were able to form functional synapses, even though their postnatal viability was limited. However, during spontaneous network activity, branched, tubular plasma membrane invaginations accumulated, capped by clathrin-coated pits, in synapses of dynamin 1-knockout mice. Synaptic vesicle endocytosis was severely impaired during strong exogenous stimulation but resumed efficiently when the stimulus was terminated. Thus, dynamin 1-independent mechanisms can support limited synaptic vesicle endocytosis, but dynamin 1 is needed during high levels of neuronal activity.

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A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis

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