A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis

Shawn M. Ferguson, Gabor Brasnjo, Mitsuko Hayashi, Markus Wölfel, Chiara Collesi, Silvia Giovedi, Andrea Raimondi, Liang Wei Gong, Pablo Ariel, Summer Paradise, Eileen O'Toole, Richard Flavell, Ottavio Cremona, Gero Miesenböck, Timothy A. Ryan, Pietro De Camilli

Research output: Contribution to journalArticle

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Abstract

Dynamin 1 is a neuron-specific guanosine triphosphatase thought to be critically required for the fission reaction of synaptic vesicle endocytosis. Unexpectedly, mice lacking dynamin 1 were able to form functional synapses, even though their postnatal viability was limited. However, during spontaneous network activity, branched, tubular plasma membrane invaginations accumulated, capped by clathrin-coated pits, in synapses of dynamin 1-knockout mice. Synaptic vesicle endocytosis was severely impaired during strong exogenous stimulation but resumed efficiently when the stimulus was terminated. Thus, dynamin 1-independent mechanisms can support limited synaptic vesicle endocytosis, but dynamin 1 is needed during high levels of neuronal activity.

Original languageEnglish
Pages (from-to)570-574
Number of pages5
JournalScience
Volume316
Issue number5824
DOIs
Publication statusPublished - Apr 27 2007

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Dynamin I
Synaptic Vesicles
Endocytosis
Synapses
Clathrin
Guanosine
Knockout Mice
Cell Membrane
Neurons

ASJC Scopus subject areas

  • General

Cite this

Ferguson, S. M., Brasnjo, G., Hayashi, M., Wölfel, M., Collesi, C., Giovedi, S., ... De Camilli, P. (2007). A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis. Science, 316(5824), 570-574. https://doi.org/10.1126/science.1140621

A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis. / Ferguson, Shawn M.; Brasnjo, Gabor; Hayashi, Mitsuko; Wölfel, Markus; Collesi, Chiara; Giovedi, Silvia; Raimondi, Andrea; Gong, Liang Wei; Ariel, Pablo; Paradise, Summer; O'Toole, Eileen; Flavell, Richard; Cremona, Ottavio; Miesenböck, Gero; Ryan, Timothy A.; De Camilli, Pietro.

In: Science, Vol. 316, No. 5824, 27.04.2007, p. 570-574.

Research output: Contribution to journalArticle

Ferguson, SM, Brasnjo, G, Hayashi, M, Wölfel, M, Collesi, C, Giovedi, S, Raimondi, A, Gong, LW, Ariel, P, Paradise, S, O'Toole, E, Flavell, R, Cremona, O, Miesenböck, G, Ryan, TA & De Camilli, P 2007, 'A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis', Science, vol. 316, no. 5824, pp. 570-574. https://doi.org/10.1126/science.1140621
Ferguson SM, Brasnjo G, Hayashi M, Wölfel M, Collesi C, Giovedi S et al. A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis. Science. 2007 Apr 27;316(5824):570-574. https://doi.org/10.1126/science.1140621
Ferguson, Shawn M. ; Brasnjo, Gabor ; Hayashi, Mitsuko ; Wölfel, Markus ; Collesi, Chiara ; Giovedi, Silvia ; Raimondi, Andrea ; Gong, Liang Wei ; Ariel, Pablo ; Paradise, Summer ; O'Toole, Eileen ; Flavell, Richard ; Cremona, Ottavio ; Miesenböck, Gero ; Ryan, Timothy A. ; De Camilli, Pietro. / A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis. In: Science. 2007 ; Vol. 316, No. 5824. pp. 570-574.
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