A sensitive and effective proteomic approach to identify she-donkey's and goat's milk adulterations by MALDI-TOF MS fingerprinting

Francesco Di Girolamo, Andrea Masotti, Guglielmo Salvatori, Margherita Scapaticci, Maurizio Muraca, Lorenza Putignani

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

She-donkey's milk (DM) and goat's milk (GM) are commonly used in newborn and infant feeding because they are less allergenic than other milk types. It is, therefore, mandatory to avoid adulteration and contamination by other milk allergens, developing fast and efficient analytical methods to assess the authenticity of these precious nutrients. In this experimental work, a sensitive and robust matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiling was designed to assess the genuineness of DM and GM milks. This workflow allows the identification of DM and GM adulteration at levels of 0.5%, thus, representing a sensitive tool for milk adulteration analysis, if compared with other laborious and time-consuming analytical procedures.

Original languageEnglish
Pages (from-to)13697-13719
Number of pages23
JournalInternational Journal of Molecular Sciences
Volume15
Issue number8
DOIs
Publication statusPublished - Aug 8 2014

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goats
milk
Equidae
Goats
Proteomics
Ionization
Mass spectrometry
Desorption
Mass Spectrometry
Milk
Lasers
mass spectroscopy
desorption
ionization
matrices
lasers
Allergens
Workflow
nutrients
Nutrients

Keywords

  • Adulteration
  • MALDI-TOF MS
  • Milk

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Spectroscopy
  • Inorganic Chemistry
  • Catalysis
  • Molecular Biology
  • Computer Science Applications
  • Medicine(all)

Cite this

A sensitive and effective proteomic approach to identify she-donkey's and goat's milk adulterations by MALDI-TOF MS fingerprinting. / Di Girolamo, Francesco; Masotti, Andrea; Salvatori, Guglielmo; Scapaticci, Margherita; Muraca, Maurizio; Putignani, Lorenza.

In: International Journal of Molecular Sciences, Vol. 15, No. 8, 08.08.2014, p. 13697-13719.

Research output: Contribution to journalArticle

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