A sensitive continuous-flow bioluminescent system for determining ethanol in serum and saliva

S. Girotti, A. Roda, S. Ghini, A. L. Piacentini, G. Carrea, R. Bovara

Research output: Contribution to journalArticle

Abstract

A continuous-flow system, based on NAD(P)H:FMN oxidoreductase and bacterial luciferase co-immobilized on a nylon coil placed in front of a photomultiplier, and alcohol dehydrogenase separately immobilized on a second nylon coil, is described for the assay of ethanol in serum and saliva. The flow is air-segmented and 5-50-μl samples are required. The sample throughput is 25-30 h-1 with no carryover. The detection limit is 1 μmol l-1 ethanol (50 pmol injected) and the response is linear between 50 and 2500 pmol of ethanol. The relative standard deviation is 3-10% for both within-day and between-day assays, and serum and saliva can be analyzed directly. The immobilized enzymes are satisfactorily stable and up to 900 samples can be analyzed with one enzyme reactor.

Original languageEnglish
Pages (from-to)187-196
Number of pages10
JournalAnalytica Chimica Acta
Volume183
Issue numberC
DOIs
Publication statusPublished - 1986

Fingerprint

saliva
Saliva
serum
ethanol
Ethanol
Nylons
Assays
FMN Reductase
Bacterial Luciferases
Serum
assay
enzyme
Immobilized Enzymes
Alcohol Dehydrogenase
Photomultipliers
airflow
Limit of Detection
alcohol
Air
Throughput

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

Cite this

Girotti, S., Roda, A., Ghini, S., Piacentini, A. L., Carrea, G., & Bovara, R. (1986). A sensitive continuous-flow bioluminescent system for determining ethanol in serum and saliva. Analytica Chimica Acta, 183(C), 187-196. https://doi.org/10.1016/0003-2670(86)80086-1

A sensitive continuous-flow bioluminescent system for determining ethanol in serum and saliva. / Girotti, S.; Roda, A.; Ghini, S.; Piacentini, A. L.; Carrea, G.; Bovara, R.

In: Analytica Chimica Acta, Vol. 183, No. C, 1986, p. 187-196.

Research output: Contribution to journalArticle

Girotti, S, Roda, A, Ghini, S, Piacentini, AL, Carrea, G & Bovara, R 1986, 'A sensitive continuous-flow bioluminescent system for determining ethanol in serum and saliva', Analytica Chimica Acta, vol. 183, no. C, pp. 187-196. https://doi.org/10.1016/0003-2670(86)80086-1
Girotti, S. ; Roda, A. ; Ghini, S. ; Piacentini, A. L. ; Carrea, G. ; Bovara, R. / A sensitive continuous-flow bioluminescent system for determining ethanol in serum and saliva. In: Analytica Chimica Acta. 1986 ; Vol. 183, No. C. pp. 187-196.
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