A serum fucolectin isolated and characterized from sea bass Dicentrarchus labrax

M. Cammarata, M. Vazzana, C. Chinnici, N. Parrinello

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

A lectin specific for fucose and galactose was isolated by affinity chromatography on Sepharose CL-6B from the serum of Dicentrarchus labrax. The hemagglutinating activity against rabbit erythrocytes was calcium-independent, and reached its maximum at 37°C. Two protein components were found in the hemagglutinating fractions eluted from the Sepharose column. Only the 34 kDa component (DLL2) eluted from the polyacrylamide gels (SDS-PAGE) showed agglutinating activity against rabbit erythrocytes. SDS-PAGE, in non-reducing conditions, revealed a single 66 kDa protein that reacted with antibodies to the 34 kDa component. Therefore, a dimeric structure stabilized by disulfide bonds can be proposed. The Ca2+-independent fucose-binding specificity, a significant amino acid sequence homology of the N-terminal trait, and cross-reaction of eel fucolectin with antibodies to DLL2 suggest that this lectin may be included in the recently identified fucolectin family.

Original languageEnglish
Pages (from-to)196-202
Number of pages7
JournalBBA - General Subjects
Volume1528
Issue number2-3
DOIs
Publication statusPublished - Oct 3 2001

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Bass
Polyacrylamide Gel Electrophoresis
Erythrocytes
Rabbits
Affinity chromatography
Amino Acid Sequence Homology
Eels
Fucose
Antibodies
Cross Reactions
Serum
Galactose
Affinity Chromatography
Lectins
Disulfides
Sepharose
Proteins
Calcium
Amino Acids
fucose-binding lectin

Keywords

  • Dicentrarchus labrax
  • Fish
  • Fucolectin
  • Hemagglutinin
  • Serum

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

A serum fucolectin isolated and characterized from sea bass Dicentrarchus labrax. / Cammarata, M.; Vazzana, M.; Chinnici, C.; Parrinello, N.

In: BBA - General Subjects, Vol. 1528, No. 2-3, 03.10.2001, p. 196-202.

Research output: Contribution to journalArticle

Cammarata, M. ; Vazzana, M. ; Chinnici, C. ; Parrinello, N. / A serum fucolectin isolated and characterized from sea bass Dicentrarchus labrax. In: BBA - General Subjects. 2001 ; Vol. 1528, No. 2-3. pp. 196-202.
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