A severe outbreak of listeriosis in central italy with a rare pulsotype associated with processed pork products

Anna Duranti, Michela Sabbatucci, Giuliana Blasi, Vicdalia Aniela Acciari, Massimo Ancora, Antonino Bella, Luca Busani, Patrizia Centorame, Cesare Camma, Fabrizio Conti, Dario De Medici, Marco Di Domenico, Violeta Di Marzio, Giovanni Filippini, Alfonsina Fiore, Stefano Fisichella, Antonietta Gattuso, Monica Gianfranceschi, Caterina Graziani, Fabrizia GuidiMaurilia Marcacci, Cristina Marfoglia, Diana Neri, Massimiliano Orsini, Donatella Ottaviani, Annalisa Petruzzelli, Patrizio Pezzotti, Caterina Rizzo, Anna Ruolo, Gaia Scavia, Stefania Scuota, Giuliano Tagliavento, Alberto Tibaldi, Francesco Tonucci, Marina Torresi, Giacomo Migliorati, Francesco Pomilio

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Purpose. From May 2015 to March 2016, an outbreak due to Listeria monocytogenes serotype 1/2a and clinical pulsotype never previously isolated in Europe occurred in central Italy, involving 24 confirmed clinical cases. The article provides a description of the outbreak and the investigation carried out by a multidisciplinary network. Methodology. Epidemiological and microbiological surveillance was conducted to confirm the outbreak and to detect the food vehicle of infection. The origin and destination of the implicated food and its ingredients were investigated by tracing-back and-forward investigation. Results. Next-generation sequencing confirmed the unique outbreak strain. On 4 January 2016, a L. monocytogenes strain with pulsotype indistinguishable from that isolated from clinical cases in the outbreak was detected in a sample of hog head cheese purchased from a retail supermarket by one of the patients. The hog head cheese was produced by a small meat processing plant in the Marche region, where microbiological investigation confirmed environmental and food contamination by the outbreak strain. Plant production was suspended and all contaminated batches of the hog head cheese were withdrawn from the market by 19 February by local health authority. We subsequently observed a sharp decline in clinical cases, the last being reported on 11 March 2016. Conclusion. The key factor in the timely conclusion of this investigation was intersectoral collaboration among epidemiologists, microbiologists, veterinarians, statisticians and health and food safety authorities at national, regional and local levels.

Original languageEnglish
Article number000785
Pages (from-to)1351-1360
Number of pages10
JournalJournal of Medical Microbiology
Volume67
Issue number9
DOIs
Publication statusPublished - Sep 1 2018

Fingerprint

Listeriosis
Italy
Disease Outbreaks
Cheese
Head
Listeria monocytogenes
Food Contamination
Food
Veterinarians
Food Safety
Health
Meat
Red Meat
Infection

Keywords

  • Bacteria
  • Bacterial meningitis
  • Food borne
  • Listeria Monocytogenes
  • Listeriosis
  • Serotype 1/2a

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

A severe outbreak of listeriosis in central italy with a rare pulsotype associated with processed pork products. / Duranti, Anna; Sabbatucci, Michela; Blasi, Giuliana; Acciari, Vicdalia Aniela; Ancora, Massimo; Bella, Antonino; Busani, Luca; Centorame, Patrizia; Camma, Cesare; Conti, Fabrizio; De Medici, Dario; Di Domenico, Marco; Di Marzio, Violeta; Filippini, Giovanni; Fiore, Alfonsina; Fisichella, Stefano; Gattuso, Antonietta; Gianfranceschi, Monica; Graziani, Caterina; Guidi, Fabrizia; Marcacci, Maurilia; Marfoglia, Cristina; Neri, Diana; Orsini, Massimiliano; Ottaviani, Donatella; Petruzzelli, Annalisa; Pezzotti, Patrizio; Rizzo, Caterina; Ruolo, Anna; Scavia, Gaia; Scuota, Stefania; Tagliavento, Giuliano; Tibaldi, Alberto; Tonucci, Francesco; Torresi, Marina; Migliorati, Giacomo; Pomilio, Francesco.

In: Journal of Medical Microbiology, Vol. 67, No. 9, 000785, 01.09.2018, p. 1351-1360.

Research output: Contribution to journalArticle

Duranti, A, Sabbatucci, M, Blasi, G, Acciari, VA, Ancora, M, Bella, A, Busani, L, Centorame, P, Camma, C, Conti, F, De Medici, D, Di Domenico, M, Di Marzio, V, Filippini, G, Fiore, A, Fisichella, S, Gattuso, A, Gianfranceschi, M, Graziani, C, Guidi, F, Marcacci, M, Marfoglia, C, Neri, D, Orsini, M, Ottaviani, D, Petruzzelli, A, Pezzotti, P, Rizzo, C, Ruolo, A, Scavia, G, Scuota, S, Tagliavento, G, Tibaldi, A, Tonucci, F, Torresi, M, Migliorati, G & Pomilio, F 2018, 'A severe outbreak of listeriosis in central italy with a rare pulsotype associated with processed pork products', Journal of Medical Microbiology, vol. 67, no. 9, 000785, pp. 1351-1360. https://doi.org/10.1099/jmm.0.000785
Duranti, Anna ; Sabbatucci, Michela ; Blasi, Giuliana ; Acciari, Vicdalia Aniela ; Ancora, Massimo ; Bella, Antonino ; Busani, Luca ; Centorame, Patrizia ; Camma, Cesare ; Conti, Fabrizio ; De Medici, Dario ; Di Domenico, Marco ; Di Marzio, Violeta ; Filippini, Giovanni ; Fiore, Alfonsina ; Fisichella, Stefano ; Gattuso, Antonietta ; Gianfranceschi, Monica ; Graziani, Caterina ; Guidi, Fabrizia ; Marcacci, Maurilia ; Marfoglia, Cristina ; Neri, Diana ; Orsini, Massimiliano ; Ottaviani, Donatella ; Petruzzelli, Annalisa ; Pezzotti, Patrizio ; Rizzo, Caterina ; Ruolo, Anna ; Scavia, Gaia ; Scuota, Stefania ; Tagliavento, Giuliano ; Tibaldi, Alberto ; Tonucci, Francesco ; Torresi, Marina ; Migliorati, Giacomo ; Pomilio, Francesco. / A severe outbreak of listeriosis in central italy with a rare pulsotype associated with processed pork products. In: Journal of Medical Microbiology. 2018 ; Vol. 67, No. 9. pp. 1351-1360.
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abstract = "Purpose. From May 2015 to March 2016, an outbreak due to Listeria monocytogenes serotype 1/2a and clinical pulsotype never previously isolated in Europe occurred in central Italy, involving 24 confirmed clinical cases. The article provides a description of the outbreak and the investigation carried out by a multidisciplinary network. Methodology. Epidemiological and microbiological surveillance was conducted to confirm the outbreak and to detect the food vehicle of infection. The origin and destination of the implicated food and its ingredients were investigated by tracing-back and-forward investigation. Results. Next-generation sequencing confirmed the unique outbreak strain. On 4 January 2016, a L. monocytogenes strain with pulsotype indistinguishable from that isolated from clinical cases in the outbreak was detected in a sample of hog head cheese purchased from a retail supermarket by one of the patients. The hog head cheese was produced by a small meat processing plant in the Marche region, where microbiological investigation confirmed environmental and food contamination by the outbreak strain. Plant production was suspended and all contaminated batches of the hog head cheese were withdrawn from the market by 19 February by local health authority. We subsequently observed a sharp decline in clinical cases, the last being reported on 11 March 2016. Conclusion. The key factor in the timely conclusion of this investigation was intersectoral collaboration among epidemiologists, microbiologists, veterinarians, statisticians and health and food safety authorities at national, regional and local levels.",
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T1 - A severe outbreak of listeriosis in central italy with a rare pulsotype associated with processed pork products

AU - Duranti, Anna

AU - Sabbatucci, Michela

AU - Blasi, Giuliana

AU - Acciari, Vicdalia Aniela

AU - Ancora, Massimo

AU - Bella, Antonino

AU - Busani, Luca

AU - Centorame, Patrizia

AU - Camma, Cesare

AU - Conti, Fabrizio

AU - De Medici, Dario

AU - Di Domenico, Marco

AU - Di Marzio, Violeta

AU - Filippini, Giovanni

AU - Fiore, Alfonsina

AU - Fisichella, Stefano

AU - Gattuso, Antonietta

AU - Gianfranceschi, Monica

AU - Graziani, Caterina

AU - Guidi, Fabrizia

AU - Marcacci, Maurilia

AU - Marfoglia, Cristina

AU - Neri, Diana

AU - Orsini, Massimiliano

AU - Ottaviani, Donatella

AU - Petruzzelli, Annalisa

AU - Pezzotti, Patrizio

AU - Rizzo, Caterina

AU - Ruolo, Anna

AU - Scavia, Gaia

AU - Scuota, Stefania

AU - Tagliavento, Giuliano

AU - Tibaldi, Alberto

AU - Tonucci, Francesco

AU - Torresi, Marina

AU - Migliorati, Giacomo

AU - Pomilio, Francesco

PY - 2018/9/1

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KW - Bacterial meningitis

KW - Food borne

KW - Listeria Monocytogenes

KW - Listeriosis

KW - Serotype 1/2a

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