A simple and rapid HPLC-UV method for the determination of retigabine in human plasma

Valentina Franco, Katia Baruffi, Giuliana Gatti, Roberto Marchiselli, Cinzia Fattore, Maria Paola Canevini, Francesca Crema, Emilio Perucca

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A simple and rapid high-performance liquid chromatographic method with ultraviolet detection was developed for the quantitative determination of retigabine, known also as ezogabine, in human plasma. The assay uses a simple solid-phase extraction for sample preparation and direct injection of the extract into the chromatograph. Flupirtine is used as an internal standard. Chromatographic separation is achieved on a C18Chromolith column (Chromolith Performance, 100 × 4.6 mm i.d.), using as mobile phase water/acetonitrile/methanol (72:18:10 v/v/v) mixed with 0.1% of 85% phosphoric acid. Isocratic elution is conducted at a flow rate of 1.5 mL min-1. The total duration of a chromatographic run is 7 min. Calibration curves are linear over the 25-2000 ng mL-1concentration range, with a limit of quantitation of 25 ng mL-1. Other performance characteristics include high precision (intra- and inter-day coefficients of variation ≤12.6%) and high accuracy (99.7%-108.7%). The method is suitable for the investigation of concentration-response relationships in patients receiving therapeutic doses of retigabine.

Original languageEnglish
JournalBiomedical Chromatography
DOIs
Publication statusE-pub ahead of print - Dec 12 2017

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Plasma (human)
flupirtine
High Pressure Liquid Chromatography
Solid Phase Extraction
Direct injection
Calibration
Methanol
Assays
Flow rate
Injections
Water
Liquids
ezogabine
Therapeutics

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A simple and rapid HPLC-UV method for the determination of retigabine in human plasma. / Franco, Valentina; Baruffi, Katia; Gatti, Giuliana; Marchiselli, Roberto; Fattore, Cinzia; Canevini, Maria Paola; Crema, Francesca; Perucca, Emilio.

In: Biomedical Chromatography, 12.12.2017.

Research output: Contribution to journalArticle

Franco, Valentina ; Baruffi, Katia ; Gatti, Giuliana ; Marchiselli, Roberto ; Fattore, Cinzia ; Canevini, Maria Paola ; Crema, Francesca ; Perucca, Emilio. / A simple and rapid HPLC-UV method for the determination of retigabine in human plasma. In: Biomedical Chromatography. 2017.
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abstract = "A simple and rapid high-performance liquid chromatographic method with ultraviolet detection was developed for the quantitative determination of retigabine, known also as ezogabine, in human plasma. The assay uses a simple solid-phase extraction for sample preparation and direct injection of the extract into the chromatograph. Flupirtine is used as an internal standard. Chromatographic separation is achieved on a C18Chromolith column (Chromolith Performance, 100 × 4.6 mm i.d.), using as mobile phase water/acetonitrile/methanol (72:18:10 v/v/v) mixed with 0.1{\%} of 85{\%} phosphoric acid. Isocratic elution is conducted at a flow rate of 1.5 mL min-1. The total duration of a chromatographic run is 7 min. Calibration curves are linear over the 25-2000 ng mL-1concentration range, with a limit of quantitation of 25 ng mL-1. Other performance characteristics include high precision (intra- and inter-day coefficients of variation ≤12.6{\%}) and high accuracy (99.7{\%}-108.7{\%}). The method is suitable for the investigation of concentration-response relationships in patients receiving therapeutic doses of retigabine.",
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AU - Canevini, Maria Paola

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AU - Perucca, Emilio

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