TY - JOUR
T1 - A simple extraction method useful to purify DNA from difficult biologic sources
AU - Oliveri, Claudio
AU - Frequin, Melissa
AU - Malferrari, Giulia
AU - Saltini, Giuliana
AU - Gramegna, Maurizio
AU - Tagliabue, Roberta
AU - De Blasio, Pasquale
AU - Biunno, Ida
AU - Biagiotti, Laura
PY - 2006
Y1 - 2006
N2 - Several procedures to isolate DNA from difficult sources have previously been described, but they are often expensive, time consuming, and have limited applications. In this paper we describe a simple and versatile protocol to isolate nucleic acids from different plant tissues, using a silica-based extraction method. This extraction process efficiently purifies DNA from several plant cells. The obtained DNA has successfully been applied in polymerase chain reaction (PCR) assays for OGM purposes. The protocol is compatible with at least two automated liquid handling systems making it suitable for large-scale screening applications.
AB - Several procedures to isolate DNA from difficult sources have previously been described, but they are often expensive, time consuming, and have limited applications. In this paper we describe a simple and versatile protocol to isolate nucleic acids from different plant tissues, using a silica-based extraction method. This extraction process efficiently purifies DNA from several plant cells. The obtained DNA has successfully been applied in polymerase chain reaction (PCR) assays for OGM purposes. The protocol is compatible with at least two automated liquid handling systems making it suitable for large-scale screening applications.
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U2 - 10.1089/cpt.2006.4.51
DO - 10.1089/cpt.2006.4.51
M3 - Article
AN - SCOPUS:33748795814
VL - 4
SP - 51
EP - 54
JO - Cell Preservation Technology
JF - Cell Preservation Technology
SN - 1538-344X
IS - 1
ER -