A simple extraction method useful to purify DNA from difficult biologic sources

Claudio Oliveri, Melissa Frequin, Giulia Malferrari, Giuliana Saltini, Maurizio Gramegna, Roberta Tagliabue, Pasquale De Blasio, Ida Biunno, Laura Biagiotti

Research output: Contribution to journalArticlepeer-review

Abstract

Several procedures to isolate DNA from difficult sources have previously been described, but they are often expensive, time consuming, and have limited applications. In this paper we describe a simple and versatile protocol to isolate nucleic acids from different plant tissues, using a silica-based extraction method. This extraction process efficiently purifies DNA from several plant cells. The obtained DNA has successfully been applied in polymerase chain reaction (PCR) assays for OGM purposes. The protocol is compatible with at least two automated liquid handling systems making it suitable for large-scale screening applications.

Original languageEnglish
Pages (from-to)51-54
Number of pages4
JournalCell Preservation Technology
Volume4
Issue number1
DOIs
Publication statusPublished - 2006

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biotechnology

Fingerprint Dive into the research topics of 'A simple extraction method useful to purify DNA from difficult biologic sources'. Together they form a unique fingerprint.

Cite this