A simple extraction method useful to purify DNA from difficult biologic sources

Claudio Oliveri; Melissa Frequin; Giulia Malferrari; Giuliana Saltini; Maurizio Gramegna; Roberta Tagliabue; Pasquale De Blasio; Ida Biunno; Laura Biagiotti

doi:10.1089/cpt.2006.4.51

A simple extraction method useful to purify DNA from difficult biologic sources

Claudio Oliveri, Melissa Frequin, Giulia Malferrari, Giuliana Saltini, Maurizio Gramegna, Roberta Tagliabue, Pasquale De Blasio, Ida Biunno, Laura Biagiotti

IRCCS Multimedica

Research output: Contribution to journal › Article › peer-review

Abstract

Several procedures to isolate DNA from difficult sources have previously been described, but they are often expensive, time consuming, and have limited applications. In this paper we describe a simple and versatile protocol to isolate nucleic acids from different plant tissues, using a silica-based extraction method. This extraction process efficiently purifies DNA from several plant cells. The obtained DNA has successfully been applied in polymerase chain reaction (PCR) assays for OGM purposes. The protocol is compatible with at least two automated liquid handling systems making it suitable for large-scale screening applications.

Original language	English
Pages (from-to)	51-54
Number of pages	4
Journal	Cell Preservation Technology
Volume	4
Issue number	1
DOIs	https://doi.org/10.1089/cpt.2006.4.51
Publication status	Published - 2006

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Biotechnology

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A simple extraction method useful to purify DNA from difficult biologic sources. / Oliveri, Claudio; Frequin, Melissa; Malferrari, Giulia; Saltini, Giuliana; Gramegna, Maurizio; Tagliabue, Roberta; De Blasio, Pasquale; Biunno, Ida; Biagiotti, Laura.

In: Cell Preservation Technology, Vol. 4, No. 1, 2006, p. 51-54.

Research output: Contribution to journal › Article › peer-review

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