A simple hemolytic micromethod for the quantitative and kinetic evaluation of the alternative activating pathway of complement

Luici D. Notarangelo, Alessandro Plebani, Massimo Marconi, Alberto Chiara, Alberto Martini, Alberto G. Ugazio

Research output: Contribution to journalArticlepeer-review

Abstract

A simple, rapid and sensitive hemolytic micromethod for quantitative and kinetic assessment of the alternative pathway of complement activity is described. The test was performed in microtitre plates using a multiscan photometer for ELISA and permits the simultaneous evaluation of as many as 20 different serum samples.

Original languageEnglish
Pages (from-to)423-429
Number of pages7
JournalLa Ricerca in Clinica e in Laboratorio
Volume13
Issue number4
DOIs
Publication statusPublished - Oct 1983

Keywords

  • Agammaglobulinemia
  • Alternative activating pathway of complement
  • C3d levels
  • CH and tH evaluations
  • Hemolytic micromethod
  • Kinetic studies
  • Severe combined immunodeficiency
  • Systemic lupus erythematosus
  • Thalassemia
  • Vasculitis

ASJC Scopus subject areas

  • Clinical Biochemistry

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