A simple method for overcoming some problems when observing thick reflective biological samples with a confocal scanning laser microscope

C. Rumio, M. Morini, A. Miani, I. Barajon, P. Castano

Research output: Contribution to journalArticlepeer-review

Abstract

A simple device is described, which allows the range of depth of scanning to be reduced when observing thick reflecting biological samples with a confocal scanning laser microscope (CSLM). Thick histological sections of human skin and rat brain stem were mounted between two coverslips ('sandwich' style) and the optical tomography was performed from both sides by turning the 'sandwich' upside-down. The samples were impregnated using standard Golgi-Cox, 'rapid Golgi' or other silver methods. The ability to turn the 'sandwich' upside-down is particularly useful when the reflective structure inspected is deep inside the section, i.e. near the lower surface of the specimen, or when it is opaque to the laser beam or excessively reflective.

Original languageEnglish
Pages (from-to)85-89
Number of pages5
JournalJournal of Microscopy
Volume177
Issue number1
Publication statusPublished - 1995

Keywords

  • confocal scanning laser microscopy
  • double coverslip mount
  • mounting
  • reflective samples

ASJC Scopus subject areas

  • Instrumentation

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