A simple method of image analysis to estimate CAM vascularization by APERIO imagescope software

Christian Marinaccio, Domenico Ribatti

Research output: Contribution to journalArticle

Abstract

The chick chorioallantoic membrane (CAM) assay is a well-established method to test the angiogenic stimulation or inhibition induced by molecules and cells administered onto the CAM. The quantification of blood vessels in the CAM assay relies on a semi-manual image analysis approach which can be time consuming when considering large experimental groups. Therefore we present here a simple and fast volumetric method to inspect differences in vascularization between experimental conditions related to the stimulation and inhibition of CAM angiogenesis based on the Positive Pixel Count algorithm embedded in the APERIO ImageScope software.

Original languageEnglish
Pages (from-to)217-219
Number of pages3
JournalInternational Journal of Developmental Biology
Volume59
Issue number4-6
DOIs
Publication statusPublished - 2015

Keywords

  • Angiogenesis
  • Chick chorioallantoic membrane assay
  • Image analysis

ASJC Scopus subject areas

  • Developmental Biology
  • Embryology

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