TY - JOUR
T1 - A simple method to measure renal function in swine by the plasma clearance of iohexol
AU - Luis-Lima, Sergio
AU - García-Contreras, Consolación
AU - Vázquez-Gómez, Marta
AU - Astiz, Susana
AU - Carrara, Fabiola
AU - Gaspari, Flavio
AU - Negrín-Mena, Natalia
AU - Jiménez-Sosa, Alejandro
AU - Jiménez-Hernández, Hugo
AU - González-Bulnes, Antonio
AU - Porrini, Esteban
PY - 2018/1/12
Y1 - 2018/1/12
N2 - There is no simplemethod tomeasure glomerular filtration rate (GFR) in swine, an established model for studying renal disease. We developed a protocol to measure GFR in conscious swine by using the plasma clearance of iohexol. We used two groups, test and validation, with eight animals each. Ten milliliters of iohexol (6.47 g) was injected into the marginal auricular vein and blood samples (3 mL) were collected from the orbital sinus at different points after injection. GFR was determined using two models: two-compartment (CL2: all samples) and one-compartment (CL1: the last six samples). In the test group, CL1 overestimated CL2 by ~30%: CL2 = 245 ± 93 and CL1 = 308 ± 123 mL/min. This error was corrected by a first-order polynomial quadratic equation to CL1, which was considered the simplified method: SM = –47.909 + (1.176xCL1) – (0.00063968xCL12). The SM showed narrow limits of agreement with CL2, a concordance correlation of 0.97, and a total deviation index of 14.73%. Similar results were obtained for the validation group. This protocol is reliable, reproducible, can be performed in conscious animals, uses a single dose of the marker, and requires a reduced number of samples, and avoids urine collection. Finally, it presents a significant improvement in animal welfare conditions and handling necessities in experimental trials.
AB - There is no simplemethod tomeasure glomerular filtration rate (GFR) in swine, an established model for studying renal disease. We developed a protocol to measure GFR in conscious swine by using the plasma clearance of iohexol. We used two groups, test and validation, with eight animals each. Ten milliliters of iohexol (6.47 g) was injected into the marginal auricular vein and blood samples (3 mL) were collected from the orbital sinus at different points after injection. GFR was determined using two models: two-compartment (CL2: all samples) and one-compartment (CL1: the last six samples). In the test group, CL1 overestimated CL2 by ~30%: CL2 = 245 ± 93 and CL1 = 308 ± 123 mL/min. This error was corrected by a first-order polynomial quadratic equation to CL1, which was considered the simplified method: SM = –47.909 + (1.176xCL1) – (0.00063968xCL12). The SM showed narrow limits of agreement with CL2, a concordance correlation of 0.97, and a total deviation index of 14.73%. Similar results were obtained for the validation group. This protocol is reliable, reproducible, can be performed in conscious animals, uses a single dose of the marker, and requires a reduced number of samples, and avoids urine collection. Finally, it presents a significant improvement in animal welfare conditions and handling necessities in experimental trials.
KW - Iohexol plasma clearance
KW - Renal function
KW - Swine model
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U2 - 10.3390/ijms19010232
DO - 10.3390/ijms19010232
M3 - Article
C2 - 29329247
AN - SCOPUS:85040948082
VL - 19
SP - 232
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1661-6596
IS - 1
ER -