A simple method to measure renal function in swine by the plasma clearance of iohexol

Sergio Luis-Lima, Consolación García-Contreras, Marta Vázquez-Gómez, Susana Astiz, Fabiola Carrara, Flavio Gaspari, Natalia Negrín-Mena, Alejandro Jiménez-Sosa, Hugo Jiménez-Hernández, Antonio González-Bulnes, Esteban Porrini

Research output: Contribution to journalArticle

Abstract

There is no simplemethod tomeasure glomerular filtration rate (GFR) in swine, an established model for studying renal disease. We developed a protocol to measure GFR in conscious swine by using the plasma clearance of iohexol. We used two groups, test and validation, with eight animals each. Ten milliliters of iohexol (6.47 g) was injected into the marginal auricular vein and blood samples (3 mL) were collected from the orbital sinus at different points after injection. GFR was determined using two models: two-compartment (CL2: all samples) and one-compartment (CL1: the last six samples). In the test group, CL1 overestimated CL2 by ~30%: CL2 = 245 ± 93 and CL1 = 308 ± 123 mL/min. This error was corrected by a first-order polynomial quadratic equation to CL1, which was considered the simplified method: SM = –47.909 + (1.176xCL1) – (0.00063968xCL12). The SM showed narrow limits of agreement with CL2, a concordance correlation of 0.97, and a total deviation index of 14.73%. Similar results were obtained for the validation group. This protocol is reliable, reproducible, can be performed in conscious animals, uses a single dose of the marker, and requires a reduced number of samples, and avoids urine collection. Finally, it presents a significant improvement in animal welfare conditions and handling necessities in experimental trials.

Original languageEnglish
Pages (from-to)232
JournalInternational Journal of Molecular Sciences
Volume19
Issue number1
DOIs
Publication statusPublished - Jan 12 2018

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Keywords

  • Iohexol plasma clearance
  • Renal function
  • Swine model

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Luis-Lima, S., García-Contreras, C., Vázquez-Gómez, M., Astiz, S., Carrara, F., Gaspari, F., Negrín-Mena, N., Jiménez-Sosa, A., Jiménez-Hernández, H., González-Bulnes, A., & Porrini, E. (2018). A simple method to measure renal function in swine by the plasma clearance of iohexol. International Journal of Molecular Sciences, 19(1), 232. https://doi.org/10.3390/ijms19010232