A Single Amino Acid Substitution, Found in Mammals with Low Susceptibility to Prion Diseases, Delays Propagation of Two Prion Strains in Highly Susceptible Transgenic Mouse Models

Alicia Otero, Carlos Hedman, Natalia Fernández-Borges, Hasier Eraña, Belén Marín, Marta Monzón, Manuel A Sánchez-Martín, Romolo Nonno, Juan José Badiola, Rosa Bolea, Joaquín Castilla

Research output: Contribution to journalArticlepeer-review

Abstract

Specific variations in the amino acid sequence of prion protein (PrP) are key determinants of susceptibility to prion diseases. We previously showed that an amino acid substitution specific to canids confers resistance to prion diseases when expressed in mice and demonstrated its dominant-negative protective effect against a variety of infectious prion strains of different origins and characteristics. Here, we show that expression of this single amino acid change significantly increases survival time in transgenic mice expressing bank vole cellular prion protein (PrPC), which is inherently prone to misfolding, following inoculation with two distinct prion strains (the CWD-vole strain and an atypical strain of spontaneous origin). This amino acid substitution hinders the propagation of both prion strains, even when expressed in the context of a PrPC uniquely susceptible to a wide range of prion isolates. Non-inoculated mice expressing this substitution experience spontaneous prion formation, but showing an increase in survival time comparable to that observed in mutant mice inoculated with the atypical strain. Our results underscore the importance of this PrP variant in the search for molecules with therapeutic potential against prion diseases.

Original languageEnglish
Pages (from-to)6501-6511
Number of pages11
JournalMolecular Neurobiology
Volume56
Issue number9
DOIs
Publication statusPublished - Sep 2019

Keywords

  • Amino Acid Substitution/genetics
  • Animals
  • Arvicolinae
  • Disease Models, Animal
  • Disease Susceptibility
  • Mammals/genetics
  • Mice, Transgenic
  • Prion Diseases/genetics
  • Prions/metabolism
  • Survival Analysis

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