The study of gene expression during preimpkntation embryonic development is useful to understand which are the molecular mechanisms involved in the progressive passage from a maternal to a zygotic regulation of gene expression. It is our laboratory main concern to develop techniques that would allow a more precise characterisation of the timing of transcription and amount of transcripts of genes that are well known to play an important role in the early events of preimplantation development. In this study we illustrate the use of a very sensitive protocol of semiquantitative single-cell RT-PCR that we have developed. This technique allows the simultaneous analysis of up to eight genes on a single cell. Here we show the experiments performed to validate this novel assay. As an example we have analysed the quantitative expression of Hprt, Bax, Bex-1, Cpt-1, pt-2, Oct-4, Tsix and Xist on single blastocysts.
|Number of pages||10|
|Publication status||Published - 2003|
- Gene expression
- Preimplantation development
- Retrotranscriptase-polymerase Chain Reaction
ASJC Scopus subject areas