A single polymerase chain reaction-based protocol for detecting normal and expanded alleles in myotonic dystrophy

Massimo Gennarelli, Marco Pavoni, Paola Amicucci, Giuseppe Novelli, Bruno Dallapiccola

Research output: Contribution to journalArticlepeer-review

Abstract

The myotonic dystrophy (DM) expansion varies from 50 to 4000 CTG repeats in the 3' untranslated region of the DMPK gene. Direct analysis by Southern blot, after restriction enzyme digestion of genomic DNA, is the method of choice for studying the DM mutation. A long polymerase chain reaction (PCR)formatted protocol, which involved a single genomic in vitro amplification followed by high concentration agarose gel electrophoresis and oligo-specific hybridization, was used to amplify normal alleles and DM alleles in all examined ranges of expansion (up to 3,700 CTGs) starting from a small amount of genomic DNA (≥15 pg). This method is quick, sensitive, and reproducible and reduces the cost of diagnostic laboratory processing.

Original languageEnglish
Pages (from-to)135-137
Number of pages3
JournalDiagnostic Molecular Pathology
Volume7
Issue number3
DOIs
Publication statusPublished - 1998

Keywords

  • CTG repeats
  • Long PCR
  • Myotonic dystrophy

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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