A single-step DNA extraction procedure for the detection of serum hepatitis B virus sequences by the polymerase chain reaction

Aldo Manzin, Giovanna Salvoni, Patrizia Bagnarelli, Stefano Menzo, Guido Carloni, Massimo Clementi

Research output: Contribution to journalArticle

Abstract

A rapid single-step procedure for the isolation of low molecular weight DNA using guanidinium thiocyanate and phenol as protein denaturants is described and applied for the detection of specific hepatitis B virus (HBV) DNA sequences from serum samples by the polymerase chain reaction (PCR). The novel technique is efficient and, when compared to the standard proteinase K/phenol/chloroform method has the advantage of being faster and easily adaptable to the routine processing of a high number of clinical samples by PCR and spot hybridization techniques.

Original languageEnglish
Pages (from-to)245-253
Number of pages9
JournalJournal of Virological Methods
Volume32
Issue number2-3
DOIs
Publication statusPublished - 1991

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Keywords

  • DNA extraction
  • Hepatitis B virus
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Virology

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