TY - JOUR
T1 - A species-specific DNA probe for Providencia stuartii identification
AU - Thaller, Maria Cristina
AU - Berlutti, Francesca
AU - Riccio, Maria Letizia
AU - Rossolini, Gian Maria
PY - 1992
Y1 - 1992
N2 - A DNA probe is described that can be used for identification of Providencia stuartii by means of filter hybridization assays. The probe, which is a fragment of the P. stuartii phoN gene coding for an acid phosphatase, appeared to be able to recognize only P. stuartii strains in slot-blot hybridization experiments performed with total DNA extracted from 545 strains of 64 different Gram-negative bacterial species, including all the major representatives of the family Enterobacteriaceae. Owing to the problems that may be often encountered for correct identification of P. stuartii at the species level when using commercial identification systems, this probe may result useful for fast and reliable identification of P. stuartii strains for taxonomical, epidemiological and diagnostic studies.
AB - A DNA probe is described that can be used for identification of Providencia stuartii by means of filter hybridization assays. The probe, which is a fragment of the P. stuartii phoN gene coding for an acid phosphatase, appeared to be able to recognize only P. stuartii strains in slot-blot hybridization experiments performed with total DNA extracted from 545 strains of 64 different Gram-negative bacterial species, including all the major representatives of the family Enterobacteriaceae. Owing to the problems that may be often encountered for correct identification of P. stuartii at the species level when using commercial identification systems, this probe may result useful for fast and reliable identification of P. stuartii strains for taxonomical, epidemiological and diagnostic studies.
KW - DNA probe
KW - filter hybridization
KW - Providencia stuartii
UR - http://www.scopus.com/inward/record.url?scp=0026934312&partnerID=8YFLogxK
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U2 - 10.1016/0890-8508(92)90036-W
DO - 10.1016/0890-8508(92)90036-W
M3 - Article
C2 - 1474980
AN - SCOPUS:0026934312
VL - 6
SP - 417
EP - 422
JO - Molecular and Cellular Probes
JF - Molecular and Cellular Probes
SN - 0890-8508
IS - 5
ER -