A species-specific DNA probe for Providencia stuartii identification

Maria Cristina Thaller, Francesca Berlutti, Maria Letizia Riccio, Gian Maria Rossolini

Research output: Contribution to journalArticle

Abstract

A DNA probe is described that can be used for identification of Providencia stuartii by means of filter hybridization assays. The probe, which is a fragment of the P. stuartii phoN gene coding for an acid phosphatase, appeared to be able to recognize only P. stuartii strains in slot-blot hybridization experiments performed with total DNA extracted from 545 strains of 64 different Gram-negative bacterial species, including all the major representatives of the family Enterobacteriaceae. Owing to the problems that may be often encountered for correct identification of P. stuartii at the species level when using commercial identification systems, this probe may result useful for fast and reliable identification of P. stuartii strains for taxonomical, epidemiological and diagnostic studies.

Original languageEnglish
Pages (from-to)417-422
Number of pages6
JournalMolecular and Cellular Probes
Volume6
Issue number5
DOIs
Publication statusPublished - 1992

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Keywords

  • DNA probe
  • filter hybridization
  • Providencia stuartii

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

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