A species-specific DNA probe for Providencia stuartii identification

Maria Cristina Thaller; Francesca Berlutti; Maria Letizia Riccio; Gian Maria Rossolini

doi:10.1016/0890-8508(92)90036-W

A species-specific DNA probe for Providencia stuartii identification

Maria Cristina Thaller, Francesca Berlutti, Maria Letizia Riccio, Gian Maria Rossolini

Fondazione Don Carlo Gnocchi Onlus

Research output: Contribution to journal › Article › peer-review

Abstract

A DNA probe is described that can be used for identification of Providencia stuartii by means of filter hybridization assays. The probe, which is a fragment of the P. stuartii phoN gene coding for an acid phosphatase, appeared to be able to recognize only P. stuartii strains in slot-blot hybridization experiments performed with total DNA extracted from 545 strains of 64 different Gram-negative bacterial species, including all the major representatives of the family Enterobacteriaceae. Owing to the problems that may be often encountered for correct identification of P. stuartii at the species level when using commercial identification systems, this probe may result useful for fast and reliable identification of P. stuartii strains for taxonomical, epidemiological and diagnostic studies.

Original language	English
Pages (from-to)	417-422
Number of pages	6
Journal	Molecular and Cellular Probes
Volume	6
Issue number	5
DOIs	https://doi.org/10.1016/0890-8508(92)90036-W
Publication status	Published - 1992

Keywords

DNA probe
filter hybridization
Providencia stuartii

ASJC Scopus subject areas

Cell Biology
Molecular Biology

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10.1016/0890-8508(92)90036-W

Cite this

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title = "A species-specific DNA probe for Providencia stuartii identification",

abstract = "A DNA probe is described that can be used for identification of Providencia stuartii by means of filter hybridization assays. The probe, which is a fragment of the P. stuartii phoN gene coding for an acid phosphatase, appeared to be able to recognize only P. stuartii strains in slot-blot hybridization experiments performed with total DNA extracted from 545 strains of 64 different Gram-negative bacterial species, including all the major representatives of the family Enterobacteriaceae. Owing to the problems that may be often encountered for correct identification of P. stuartii at the species level when using commercial identification systems, this probe may result useful for fast and reliable identification of P. stuartii strains for taxonomical, epidemiological and diagnostic studies.",

keywords = "DNA probe, filter hybridization, Providencia stuartii",

author = "Thaller, {Maria Cristina} and Francesca Berlutti and Riccio, {Maria Letizia} and Rossolini, {Gian Maria}",

year = "1992",

doi = "10.1016/0890-8508(92)90036-W",

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T1 - A species-specific DNA probe for Providencia stuartii identification

AU - Thaller, Maria Cristina

AU - Berlutti, Francesca

AU - Riccio, Maria Letizia

AU - Rossolini, Gian Maria

PY - 1992

Y1 - 1992

N2 - A DNA probe is described that can be used for identification of Providencia stuartii by means of filter hybridization assays. The probe, which is a fragment of the P. stuartii phoN gene coding for an acid phosphatase, appeared to be able to recognize only P. stuartii strains in slot-blot hybridization experiments performed with total DNA extracted from 545 strains of 64 different Gram-negative bacterial species, including all the major representatives of the family Enterobacteriaceae. Owing to the problems that may be often encountered for correct identification of P. stuartii at the species level when using commercial identification systems, this probe may result useful for fast and reliable identification of P. stuartii strains for taxonomical, epidemiological and diagnostic studies.

AB - A DNA probe is described that can be used for identification of Providencia stuartii by means of filter hybridization assays. The probe, which is a fragment of the P. stuartii phoN gene coding for an acid phosphatase, appeared to be able to recognize only P. stuartii strains in slot-blot hybridization experiments performed with total DNA extracted from 545 strains of 64 different Gram-negative bacterial species, including all the major representatives of the family Enterobacteriaceae. Owing to the problems that may be often encountered for correct identification of P. stuartii at the species level when using commercial identification systems, this probe may result useful for fast and reliable identification of P. stuartii strains for taxonomical, epidemiological and diagnostic studies.

KW - DNA probe

KW - filter hybridization

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A species-specific DNA probe for Providencia stuartii identification

Abstract

Keywords

ASJC Scopus subject areas

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