A specific gas chromatographic method for the determination of microsomal styrene monooxygenase and styrene epoxide hydratase activities

G. Belvedere, J. Pachecka, L. Cantoni, E. Mussini, M. Salmona

Research output: Contribution to journalArticle

Abstract

A gas chromatographic (GC) method for the determination of the metabolite resulting from the activities of microsomal styrene monooxygenase (epoxide synthetase) and epoxide hydratase using styrene or styrene epoxide as substrates has been developed. The determination of the activities of both enzymes is based on the GC determination of phenylethylene glycol after its esterification with n-butylboronic acid. Kinetic parameters for both enzymes are given.

Original languageEnglish
Pages (from-to)387-393
Number of pages7
JournalJournal of Chromatography A
Volume118
Issue number3
DOIs
Publication statusPublished - Apr 7 1976

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Aryl Hydrocarbon Hydroxylases
Epoxide Hydrolases
Styrene
Epoxy Compounds
Gases
Esterification
Enzymes
Ligases
Metabolites
Kinetic parameters
Substrates
styrene monooxygenase
n-butylboronic acid
styrene glycol

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

A specific gas chromatographic method for the determination of microsomal styrene monooxygenase and styrene epoxide hydratase activities. / Belvedere, G.; Pachecka, J.; Cantoni, L.; Mussini, E.; Salmona, M.

In: Journal of Chromatography A, Vol. 118, No. 3, 07.04.1976, p. 387-393.

Research output: Contribution to journalArticle

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