TY - JOUR
T1 - A structured chitosan-based platform for biomolecule attachment to solid surfaces
T2 - Application to DNA microarray preparation
AU - Consolandi, Clarissa
AU - Severgnini, Marco
AU - Castiglioni, Bianca
AU - Bordoni, Roberta
AU - Frosini, Andrea
AU - Battaglia, Cristina
AU - Bernardi, Luigi Rossi
AU - De Bellis, Gianluca
PY - 2006/3
Y1 - 2006/3
N2 - A structured chemical platform based on chitosan, an amine-rich polysaccharide, is presented as an alternative chemistry to functionalize solid support (in this case, glass slides) for grafting biomolecules. This approach has been adopted for generating arrays using amino-modified oligonucleotides with two different lengths (25-mer and 70-mer) for different purposes. Results using these chitosan-activated surfaces indicate high oligonucleotide loading capacity, good availability to hybridization against targets, and effectiveness in enzyme-mediated single nucleotide polymorphism (SNP) detection procedures by DNA polymerase and DNA ligase enzymes with low background. Universal arrays have been prepared and extensively used with excellent results in different applications. The chitosan-treated surfaces were also evaluated for their performance in a gene expression experiment.
AB - A structured chemical platform based on chitosan, an amine-rich polysaccharide, is presented as an alternative chemistry to functionalize solid support (in this case, glass slides) for grafting biomolecules. This approach has been adopted for generating arrays using amino-modified oligonucleotides with two different lengths (25-mer and 70-mer) for different purposes. Results using these chitosan-activated surfaces indicate high oligonucleotide loading capacity, good availability to hybridization against targets, and effectiveness in enzyme-mediated single nucleotide polymorphism (SNP) detection procedures by DNA polymerase and DNA ligase enzymes with low background. Universal arrays have been prepared and extensively used with excellent results in different applications. The chitosan-treated surfaces were also evaluated for their performance in a gene expression experiment.
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U2 - 10.1021/bc050285a
DO - 10.1021/bc050285a
M3 - Article
C2 - 16536468
AN - SCOPUS:33645466884
VL - 17
SP - 371
EP - 377
JO - Bioconjugate Chemistry
JF - Bioconjugate Chemistry
SN - 1043-1802
IS - 2
ER -