A study of cytopathic rotavirus strains isolated from calves with acute enteritis

G. Castrucci, M. Ferrari, F. Frigeri, V. Cilli, G. Donelli, G. Angelillo, M. Bruggi

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Nine cytopathic bovine rotavirus strains were isolated in MA-104 cell cultures from fecal specimens of dairy calves suffering from diarrhea. Isolation of the virus was accomplished from three outbreaks which occurred on dairy farms located in Central and Southern Italy. Fecal suspensions were treated with a high concentration (1000 μg/ml) of trypsin, and inoculated into MA-104 cell cultures grown out in Eagle's minimum essential medium (MEM) containing 5 μg/ml of the enzyme. Cytopathic effects (CPE), characterized by intracytoplasmic inclusion bodies of different sizes and shapes, were observed on the 1st passage with five of the strains and on the 2nd (2 strains) or the 3rd (2 strains) passage for the others. The presence of trypsin and the use of MA-104 cells appeared to be essential for the occurrence of CPE, inasmuch as no CPE was detected when trypsin was omitted in the MA-104 cell system. Replication failed to occur when primary bovine embryo kidney cell cultures with or without trypsin were used. Electron microscopy revealed the presence of particles with a typical rotavirus morphology. In MA-104 cells, the titre of virus reached its maximum 48 hr after inoculation. Small, clear-cut plaques were produced by the isolates in MA-104 cells under the overlay of MEM containing carboxymethylecellulose, trypsin and DEAE-dextran. The nine rotavirus strains were antigenically related, whereas the relationship to either the Nebraska or the Compton rotaviruses was quite weak.

Original languageEnglish
Pages (from-to)253-264
Number of pages12
JournalComparative Immunology, Microbiology and Infectious Diseases
Volume6
Issue number3
DOIs
Publication statusPublished - 1983

Fingerprint

Enteritis
Rotavirus
enteritis
Trypsin
trypsin
calves
cytopathogenicity
cell culture
Cell Culture Techniques
animal morphology
cells
DEAE-Dextran
Eagles
dairy calves
cattle
Inclusion Bodies
inclusion bodies
Body Size
eagles
dextran

Keywords

  • acute enteritis
  • Calves
  • cytopathic rotavirus
  • isolation

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Immunology
  • Microbiology
  • veterinary(all)

Cite this

A study of cytopathic rotavirus strains isolated from calves with acute enteritis. / Castrucci, G.; Ferrari, M.; Frigeri, F.; Cilli, V.; Donelli, G.; Angelillo, G.; Bruggi, M.

In: Comparative Immunology, Microbiology and Infectious Diseases, Vol. 6, No. 3, 1983, p. 253-264.

Research output: Contribution to journalArticle

Castrucci, G, Ferrari, M, Frigeri, F, Cilli, V, Donelli, G, Angelillo, G & Bruggi, M 1983, 'A study of cytopathic rotavirus strains isolated from calves with acute enteritis', Comparative Immunology, Microbiology and Infectious Diseases, vol. 6, no. 3, pp. 253-264. https://doi.org/10.1016/0147-9571(83)90018-8
Castrucci, G. ; Ferrari, M. ; Frigeri, F. ; Cilli, V. ; Donelli, G. ; Angelillo, G. ; Bruggi, M. / A study of cytopathic rotavirus strains isolated from calves with acute enteritis. In: Comparative Immunology, Microbiology and Infectious Diseases. 1983 ; Vol. 6, No. 3. pp. 253-264.
@article{bfeffe66f5924777b19ccc5666f1f50a,
title = "A study of cytopathic rotavirus strains isolated from calves with acute enteritis",
abstract = "Nine cytopathic bovine rotavirus strains were isolated in MA-104 cell cultures from fecal specimens of dairy calves suffering from diarrhea. Isolation of the virus was accomplished from three outbreaks which occurred on dairy farms located in Central and Southern Italy. Fecal suspensions were treated with a high concentration (1000 μg/ml) of trypsin, and inoculated into MA-104 cell cultures grown out in Eagle's minimum essential medium (MEM) containing 5 μg/ml of the enzyme. Cytopathic effects (CPE), characterized by intracytoplasmic inclusion bodies of different sizes and shapes, were observed on the 1st passage with five of the strains and on the 2nd (2 strains) or the 3rd (2 strains) passage for the others. The presence of trypsin and the use of MA-104 cells appeared to be essential for the occurrence of CPE, inasmuch as no CPE was detected when trypsin was omitted in the MA-104 cell system. Replication failed to occur when primary bovine embryo kidney cell cultures with or without trypsin were used. Electron microscopy revealed the presence of particles with a typical rotavirus morphology. In MA-104 cells, the titre of virus reached its maximum 48 hr after inoculation. Small, clear-cut plaques were produced by the isolates in MA-104 cells under the overlay of MEM containing carboxymethylecellulose, trypsin and DEAE-dextran. The nine rotavirus strains were antigenically related, whereas the relationship to either the Nebraska or the Compton rotaviruses was quite weak.",
keywords = "acute enteritis, Calves, cytopathic rotavirus, isolation",
author = "G. Castrucci and M. Ferrari and F. Frigeri and V. Cilli and G. Donelli and G. Angelillo and M. Bruggi",
year = "1983",
doi = "10.1016/0147-9571(83)90018-8",
language = "English",
volume = "6",
pages = "253--264",
journal = "Comparative Immunology, Microbiology and Infectious Diseases",
issn = "0147-9571",
publisher = "Elsevier Limited",
number = "3",

}

TY - JOUR

T1 - A study of cytopathic rotavirus strains isolated from calves with acute enteritis

AU - Castrucci, G.

AU - Ferrari, M.

AU - Frigeri, F.

AU - Cilli, V.

AU - Donelli, G.

AU - Angelillo, G.

AU - Bruggi, M.

PY - 1983

Y1 - 1983

N2 - Nine cytopathic bovine rotavirus strains were isolated in MA-104 cell cultures from fecal specimens of dairy calves suffering from diarrhea. Isolation of the virus was accomplished from three outbreaks which occurred on dairy farms located in Central and Southern Italy. Fecal suspensions were treated with a high concentration (1000 μg/ml) of trypsin, and inoculated into MA-104 cell cultures grown out in Eagle's minimum essential medium (MEM) containing 5 μg/ml of the enzyme. Cytopathic effects (CPE), characterized by intracytoplasmic inclusion bodies of different sizes and shapes, were observed on the 1st passage with five of the strains and on the 2nd (2 strains) or the 3rd (2 strains) passage for the others. The presence of trypsin and the use of MA-104 cells appeared to be essential for the occurrence of CPE, inasmuch as no CPE was detected when trypsin was omitted in the MA-104 cell system. Replication failed to occur when primary bovine embryo kidney cell cultures with or without trypsin were used. Electron microscopy revealed the presence of particles with a typical rotavirus morphology. In MA-104 cells, the titre of virus reached its maximum 48 hr after inoculation. Small, clear-cut plaques were produced by the isolates in MA-104 cells under the overlay of MEM containing carboxymethylecellulose, trypsin and DEAE-dextran. The nine rotavirus strains were antigenically related, whereas the relationship to either the Nebraska or the Compton rotaviruses was quite weak.

AB - Nine cytopathic bovine rotavirus strains were isolated in MA-104 cell cultures from fecal specimens of dairy calves suffering from diarrhea. Isolation of the virus was accomplished from three outbreaks which occurred on dairy farms located in Central and Southern Italy. Fecal suspensions were treated with a high concentration (1000 μg/ml) of trypsin, and inoculated into MA-104 cell cultures grown out in Eagle's minimum essential medium (MEM) containing 5 μg/ml of the enzyme. Cytopathic effects (CPE), characterized by intracytoplasmic inclusion bodies of different sizes and shapes, were observed on the 1st passage with five of the strains and on the 2nd (2 strains) or the 3rd (2 strains) passage for the others. The presence of trypsin and the use of MA-104 cells appeared to be essential for the occurrence of CPE, inasmuch as no CPE was detected when trypsin was omitted in the MA-104 cell system. Replication failed to occur when primary bovine embryo kidney cell cultures with or without trypsin were used. Electron microscopy revealed the presence of particles with a typical rotavirus morphology. In MA-104 cells, the titre of virus reached its maximum 48 hr after inoculation. Small, clear-cut plaques were produced by the isolates in MA-104 cells under the overlay of MEM containing carboxymethylecellulose, trypsin and DEAE-dextran. The nine rotavirus strains were antigenically related, whereas the relationship to either the Nebraska or the Compton rotaviruses was quite weak.

KW - acute enteritis

KW - Calves

KW - cytopathic rotavirus

KW - isolation

UR - http://www.scopus.com/inward/record.url?scp=0020689654&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020689654&partnerID=8YFLogxK

U2 - 10.1016/0147-9571(83)90018-8

DO - 10.1016/0147-9571(83)90018-8

M3 - Article

VL - 6

SP - 253

EP - 264

JO - Comparative Immunology, Microbiology and Infectious Diseases

JF - Comparative Immunology, Microbiology and Infectious Diseases

SN - 0147-9571

IS - 3

ER -