A study of mutations in the c-kit gene of 32 dogs with mastocytoma

Federica Riva, Stefano Brizzola, Damiano Stefanello, Simone Crema, Lauretta Turin

Research output: Contribution to journalArticle

Abstract

Mutations in the intracellular juxtamembrane domain of the c-kit gene in 32 dogs with different grades of histologically confirmed mastocytoma were studied. Transcript RNAs extracted from neoplastic tissue surgically collected from dogs of different breeds and from a negative control were reverse transcribed into complementary DNA and amplified by polymerase chain reaction. The region corresponding to the c-kit juxtamembrane domain was sequenced and compared with GenBank sequences. Two different types of mutations were identified within exon 11: a previously underscribed single-nucleotide substitution and a 6-bp deletion. The c-kit juxtamembrane domain sequences of all dogs were grouped in 3 clusters. No mutations were detected in tissues constitutively expressing c-kit (cerebellum and spleen), obtained from dogs not affected by mastocytoma (controls). All the substitutions were found in dogs bearing grade I or II mast cell tumors; the deletion was detected in 1 dog with grade II mastocytoma.

Original languageEnglish
Pages (from-to)385-388
Number of pages4
JournalJournal of Veterinary Diagnostic Investigation
Volume17
Issue number4
Publication statusPublished - Jul 2005

Keywords

  • c-kit
  • Canine
  • Dogs
  • KIT receptor
  • Mastocytoma
  • Mutation
  • Oncogene

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

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    Riva, F., Brizzola, S., Stefanello, D., Crema, S., & Turin, L. (2005). A study of mutations in the c-kit gene of 32 dogs with mastocytoma. Journal of Veterinary Diagnostic Investigation, 17(4), 385-388.