A study of the effect on human mesenchymal stem cells of an atmospheric pressure plasma source driven by different voltage waveforms

R. Laurita, F. Alviano, C. Marchionni, P. M. Abruzzo, A. Bolotta, L. Bonsi, V. Colombo, M. Gherardi, A. Liguori, F. Ricci, M. Rossi, A. Stancampiano, P. L. Tazzari, Marina Marini

Research output: Contribution to journalArticle

Abstract

The effect of an atmospheric pressure non-equilibrium plasma on human mesenchymal stem cells was investigated. A dielectric barrier discharge non-equilibrium plasma source driven by two different high-voltage pulsed generators was used and cell survival, senescence, proliferation, and differentiation were evaluated. Cells deprived of the culture medium and treated with nanosecond pulsed plasma showed a higher mortality rate, while higher survival and retention of proliferation were observed in cells treated with microsecond pulsed plasma in the presence of the culture medium. While a few treated cells showed the hallmarks of senescence, unexpected delayed apoptosis ensued in cells exposed to plasma-treated medium. The plasma treatment did not change the expression of OCT4, a marker of mesenchymal stem cell differentiation.

Original languageEnglish
Article number364003
JournalJournal of Physics D: Applied Physics
Volume49
Issue number36
DOIs
Publication statusPublished - Aug 9 2016

Fingerprint

Plasma sources
stem cells
Stem cells
Atmospheric pressure
atmospheric pressure
waveforms
Plasmas
nonequilibrium plasmas
culture media
Electric potential
electric potential
cells
Culture Media
Cells
mortality
apoptosis
markers
high voltages
Cell death
generators

Keywords

  • atmospheric pressure non-equilibrium plasma
  • dielectric barrier discharge
  • fetal membrane-derived human mesenchymal stem cells
  • high voltage pulsed discharge

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Acoustics and Ultrasonics
  • Surfaces, Coatings and Films

Cite this

A study of the effect on human mesenchymal stem cells of an atmospheric pressure plasma source driven by different voltage waveforms. / Laurita, R.; Alviano, F.; Marchionni, C.; Abruzzo, P. M.; Bolotta, A.; Bonsi, L.; Colombo, V.; Gherardi, M.; Liguori, A.; Ricci, F.; Rossi, M.; Stancampiano, A.; Tazzari, P. L.; Marini, Marina.

In: Journal of Physics D: Applied Physics, Vol. 49, No. 36, 364003, 09.08.2016.

Research output: Contribution to journalArticle

Laurita, R, Alviano, F, Marchionni, C, Abruzzo, PM, Bolotta, A, Bonsi, L, Colombo, V, Gherardi, M, Liguori, A, Ricci, F, Rossi, M, Stancampiano, A, Tazzari, PL & Marini, M 2016, 'A study of the effect on human mesenchymal stem cells of an atmospheric pressure plasma source driven by different voltage waveforms', Journal of Physics D: Applied Physics, vol. 49, no. 36, 364003. https://doi.org/10.1088/0022-3727/49/36/364003
Laurita, R. ; Alviano, F. ; Marchionni, C. ; Abruzzo, P. M. ; Bolotta, A. ; Bonsi, L. ; Colombo, V. ; Gherardi, M. ; Liguori, A. ; Ricci, F. ; Rossi, M. ; Stancampiano, A. ; Tazzari, P. L. ; Marini, Marina. / A study of the effect on human mesenchymal stem cells of an atmospheric pressure plasma source driven by different voltage waveforms. In: Journal of Physics D: Applied Physics. 2016 ; Vol. 49, No. 36.
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