A study of the intracellular effects of glutathione by 1H-spin echo NMR of intact human erythrocytes

M. R. Ciriolo, M. Sette, M. Paci, G. Rotilio

Research output: Contribution to journalArticlepeer-review

Abstract

The effect of adding either reduced (GSH) or oxidized (GSSG) glutathione to intact human erythrocytes was investigated by 1H-spin echo NMR, which allows direct observation of relatively concentrated low molecular weight compounds within intact cells. A specific region of the spectrum was affected by addition of GSH, with the appearance of new peaks that were diagnostic of an increase of intracellular GSH. These changes did not occur in hemolysates, and did not involve extra-cytosol GSH either free or membrane-bound. These results indicate that the intracellular redox balance of glutathione is shifted toward the reduced state by exogenous glutathione, possibly via a signal transferring system of the cell membrane.

Original languageEnglish
Pages (from-to)397-403
Number of pages7
JournalBiochemistry International
Volume20
Issue number2
Publication statusPublished - 1990

ASJC Scopus subject areas

  • Biochemistry

Fingerprint Dive into the research topics of 'A study of the intracellular effects of glutathione by 1H-spin echo NMR of intact human erythrocytes'. Together they form a unique fingerprint.

Cite this