A subpopulation of murine bone marrow cells fully differentiates along the myogenic pathway and participates in muscle repair in the mdx dystrophic mouse

S. Corti, S. Strazzer, R. Del Bo, S. Salani, P. Bossolasco, F. Fortunato, F. Locatelli, D. Soligo, M. Moggio, P. Ciscato, A. Prelle, C. Borsotti, N. Bresolin, G. Scarlato, G. P. Comi

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Bone marrow (BM) transplantation in mice suggests the existence of pluripotent cells able to differentiate into skeletal muscle tissue, although sustained myofiber reconstitution has not yet been achieved. We investigated the myogenic potential of mouse BM cells and evaluated whether a BM fraction enriched for cells expressing skeletal muscle markers would ameliorate muscle repair, when compared to whole BM, into the dystrophic mdx mouse. We demonstrate that cells expressing striated-muscle-specific proteins are already present in the BM independently from experimentally forced myogenic conversion. We observed the presence of both markers of early myogenic program such as Pax3, Myf5, MyoD, desmin, and late myogenesis such as myosin heavy chain and α-sarcomeric actin. These myogenic cells are more represented in the early nonadherent BM fraction, which generates clones able to fully differentiate into myotubes. Transplantation in mdx mice by intravenous injection of whole BM and a tenfold BM myogenic enriched fraction resulted in BM reconstitution and limited dystrophin restoration. Taken together, these data show that a fraction of BM cells have a definite potential for differentiation along the skeletal muscle pathway and can be recruited by muscle repair mechanisms. They also indicate that factors limiting the degree of muscle recruitment and the host stem cell competition should be assessed in order to evaluate the usefulness of BM-derived myogenic cells into the context of cell-mediated gene therapy of inherited muscle diseases.

Original languageEnglish
Pages (from-to)74-85
Number of pages12
JournalExperimental Cell Research
Volume277
Issue number1
DOIs
Publication statusPublished - 2002

Fingerprint

Inbred mdx Mouse
Bone Marrow Cells
Bone Marrow
Muscles
Skeletal Muscle
Dystrophin
Desmin
Striated Muscle
Myosin Heavy Chains
Muscle Development
Muscle Proteins
Skeletal Muscle Fibers
Bone Marrow Transplantation
Intravenous Injections
Genetic Therapy
Actins
Stem Cells
Clone Cells
Transplantation

Keywords

  • Bone marrow
  • Cell transplantation
  • Dystrophin
  • Gene therapy
  • Stem cell

ASJC Scopus subject areas

  • Cell Biology

Cite this

@article{1aeb4b6e796d49c282a216df63b81517,
title = "A subpopulation of murine bone marrow cells fully differentiates along the myogenic pathway and participates in muscle repair in the mdx dystrophic mouse",
abstract = "Bone marrow (BM) transplantation in mice suggests the existence of pluripotent cells able to differentiate into skeletal muscle tissue, although sustained myofiber reconstitution has not yet been achieved. We investigated the myogenic potential of mouse BM cells and evaluated whether a BM fraction enriched for cells expressing skeletal muscle markers would ameliorate muscle repair, when compared to whole BM, into the dystrophic mdx mouse. We demonstrate that cells expressing striated-muscle-specific proteins are already present in the BM independently from experimentally forced myogenic conversion. We observed the presence of both markers of early myogenic program such as Pax3, Myf5, MyoD, desmin, and late myogenesis such as myosin heavy chain and α-sarcomeric actin. These myogenic cells are more represented in the early nonadherent BM fraction, which generates clones able to fully differentiate into myotubes. Transplantation in mdx mice by intravenous injection of whole BM and a tenfold BM myogenic enriched fraction resulted in BM reconstitution and limited dystrophin restoration. Taken together, these data show that a fraction of BM cells have a definite potential for differentiation along the skeletal muscle pathway and can be recruited by muscle repair mechanisms. They also indicate that factors limiting the degree of muscle recruitment and the host stem cell competition should be assessed in order to evaluate the usefulness of BM-derived myogenic cells into the context of cell-mediated gene therapy of inherited muscle diseases.",
keywords = "Bone marrow, Cell transplantation, Dystrophin, Gene therapy, Stem cell",
author = "S. Corti and S. Strazzer and {Del Bo}, R. and S. Salani and P. Bossolasco and F. Fortunato and F. Locatelli and D. Soligo and M. Moggio and P. Ciscato and A. Prelle and C. Borsotti and N. Bresolin and G. Scarlato and Comi, {G. P.}",
year = "2002",
doi = "10.1006/excr.2002.5543",
language = "English",
volume = "277",
pages = "74--85",
journal = "Experimental Cell Research",
issn = "0014-4827",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - A subpopulation of murine bone marrow cells fully differentiates along the myogenic pathway and participates in muscle repair in the mdx dystrophic mouse

AU - Corti, S.

AU - Strazzer, S.

AU - Del Bo, R.

AU - Salani, S.

AU - Bossolasco, P.

AU - Fortunato, F.

AU - Locatelli, F.

AU - Soligo, D.

AU - Moggio, M.

AU - Ciscato, P.

AU - Prelle, A.

AU - Borsotti, C.

AU - Bresolin, N.

AU - Scarlato, G.

AU - Comi, G. P.

PY - 2002

Y1 - 2002

N2 - Bone marrow (BM) transplantation in mice suggests the existence of pluripotent cells able to differentiate into skeletal muscle tissue, although sustained myofiber reconstitution has not yet been achieved. We investigated the myogenic potential of mouse BM cells and evaluated whether a BM fraction enriched for cells expressing skeletal muscle markers would ameliorate muscle repair, when compared to whole BM, into the dystrophic mdx mouse. We demonstrate that cells expressing striated-muscle-specific proteins are already present in the BM independently from experimentally forced myogenic conversion. We observed the presence of both markers of early myogenic program such as Pax3, Myf5, MyoD, desmin, and late myogenesis such as myosin heavy chain and α-sarcomeric actin. These myogenic cells are more represented in the early nonadherent BM fraction, which generates clones able to fully differentiate into myotubes. Transplantation in mdx mice by intravenous injection of whole BM and a tenfold BM myogenic enriched fraction resulted in BM reconstitution and limited dystrophin restoration. Taken together, these data show that a fraction of BM cells have a definite potential for differentiation along the skeletal muscle pathway and can be recruited by muscle repair mechanisms. They also indicate that factors limiting the degree of muscle recruitment and the host stem cell competition should be assessed in order to evaluate the usefulness of BM-derived myogenic cells into the context of cell-mediated gene therapy of inherited muscle diseases.

AB - Bone marrow (BM) transplantation in mice suggests the existence of pluripotent cells able to differentiate into skeletal muscle tissue, although sustained myofiber reconstitution has not yet been achieved. We investigated the myogenic potential of mouse BM cells and evaluated whether a BM fraction enriched for cells expressing skeletal muscle markers would ameliorate muscle repair, when compared to whole BM, into the dystrophic mdx mouse. We demonstrate that cells expressing striated-muscle-specific proteins are already present in the BM independently from experimentally forced myogenic conversion. We observed the presence of both markers of early myogenic program such as Pax3, Myf5, MyoD, desmin, and late myogenesis such as myosin heavy chain and α-sarcomeric actin. These myogenic cells are more represented in the early nonadherent BM fraction, which generates clones able to fully differentiate into myotubes. Transplantation in mdx mice by intravenous injection of whole BM and a tenfold BM myogenic enriched fraction resulted in BM reconstitution and limited dystrophin restoration. Taken together, these data show that a fraction of BM cells have a definite potential for differentiation along the skeletal muscle pathway and can be recruited by muscle repair mechanisms. They also indicate that factors limiting the degree of muscle recruitment and the host stem cell competition should be assessed in order to evaluate the usefulness of BM-derived myogenic cells into the context of cell-mediated gene therapy of inherited muscle diseases.

KW - Bone marrow

KW - Cell transplantation

KW - Dystrophin

KW - Gene therapy

KW - Stem cell

UR - http://www.scopus.com/inward/record.url?scp=0036343850&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036343850&partnerID=8YFLogxK

U2 - 10.1006/excr.2002.5543

DO - 10.1006/excr.2002.5543

M3 - Article

C2 - 12061818

AN - SCOPUS:0036343850

VL - 277

SP - 74

EP - 85

JO - Experimental Cell Research

JF - Experimental Cell Research

SN - 0014-4827

IS - 1

ER -