A subset of human CD34+ hematopoietic progenitors express low levels of CD4, the high-affinity receptor for human immunodeficiency virus-type 1

Giorgio Zauli, Giuliano Furlini, Marco Vitale, Maria Carla Re, Davide Gibellini, Loris Zamai, Giuseppe Visani, Paola Borgatti, Silvano Capitani, Michele La Placa

Research output: Contribution to journalArticle

Abstract

We investigated the expression of CD4 antigen in normal bone marrow (BM) samples, enriched in CD34+ hematopoietic progenitor cells. At flow cytometry, a significant fraction (ranging from 25% to 65%) of CD34+ cells also showed low levels of CD4 antigen on their surface. The CD4 receptor densities on the surface of hematopoietic progenitors was approximately 50% that of peripheral blood monocytes and 5% of peripheral blood T lymphocytes. In immunoprecipitation experiments, the CD4 antigen expressed by BM hematopoietic progenitors appeared to be the same form expressed by mature peripheral blood CD4+ cells and appeared to be a potentially functional receptor for human immunodeficiency virus-type 1, because it specifically bound recombinant envelope gp120. Moreover, BM samples, highly enriched in CD34+ cells, showed the presence of CD4 mRNA at reverse transcription- polymerase chain reaction examination. In experiments of complement-mediated cytotoxicity with Leu3-a+Leu3b anti-CD4 monoclonal antibody, a significant reduction in the number of both classes of megakaryocyte (burst-forming unit- meg [BFU-meg] and colony-forming unit-meg [CFU-meg]) and granulocyte/macrophage (CFU-GM) progenitors was observed, whereas erythroid (BFU-E) progenitors were only slightly affected. Moreover, purified CD4+ BM cells obtained by immunomagnetic selection, using high concentrations of Leu3a+Leu3b, showed a colony-forming ability of megakaryocyte and granulocyte/macrophage progenitors comparable with that of CD4- BM cells. In conclusion, the present data show that immature hematopoietic progenitor cells express low levels of CD4, the high-affinity receptor of human immunodeficiency virus-type 1.

Original languageEnglish
Pages (from-to)1896-1905
Number of pages10
JournalBlood
Volume84
Issue number6
Publication statusPublished - Sep 15 1994

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CD4 Antigens
Viruses
Granulocyte-Macrophage Progenitor Cells
HIV-1
Bone
Megakaryocytes
Bone Marrow
Hematopoietic Stem Cells
Blood
Bone Marrow Cells
Macrophages
Erythroid Precursor Cells
Immunoprecipitation
T-cells
Flow cytometry
Reverse Transcription
Polymerase chain reaction
Monocytes
Transcription
Blood Cells

ASJC Scopus subject areas

  • Hematology

Cite this

Zauli, G., Furlini, G., Vitale, M., Re, M. C., Gibellini, D., Zamai, L., ... La Placa, M. (1994). A subset of human CD34+ hematopoietic progenitors express low levels of CD4, the high-affinity receptor for human immunodeficiency virus-type 1. Blood, 84(6), 1896-1905.

A subset of human CD34+ hematopoietic progenitors express low levels of CD4, the high-affinity receptor for human immunodeficiency virus-type 1. / Zauli, Giorgio; Furlini, Giuliano; Vitale, Marco; Re, Maria Carla; Gibellini, Davide; Zamai, Loris; Visani, Giuseppe; Borgatti, Paola; Capitani, Silvano; La Placa, Michele.

In: Blood, Vol. 84, No. 6, 15.09.1994, p. 1896-1905.

Research output: Contribution to journalArticle

Zauli, G, Furlini, G, Vitale, M, Re, MC, Gibellini, D, Zamai, L, Visani, G, Borgatti, P, Capitani, S & La Placa, M 1994, 'A subset of human CD34+ hematopoietic progenitors express low levels of CD4, the high-affinity receptor for human immunodeficiency virus-type 1', Blood, vol. 84, no. 6, pp. 1896-1905.
Zauli, Giorgio ; Furlini, Giuliano ; Vitale, Marco ; Re, Maria Carla ; Gibellini, Davide ; Zamai, Loris ; Visani, Giuseppe ; Borgatti, Paola ; Capitani, Silvano ; La Placa, Michele. / A subset of human CD34+ hematopoietic progenitors express low levels of CD4, the high-affinity receptor for human immunodeficiency virus-type 1. In: Blood. 1994 ; Vol. 84, No. 6. pp. 1896-1905.
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AU - Re, Maria Carla

AU - Gibellini, Davide

AU - Zamai, Loris

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