A T-cell diagnostic test for cystic echinococcosis based on Antigen B peptides

L. Petrone, V. Vanini, M. Amicosante, A. Corpolongo, M. A. Gomez Morales, A. Ludovisi, G. Ippolito, E. Pozio, A. Teggi, D. Goletti

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Cystic echinococcosis (CE) immunodiagnosis is still imperfect. We recently set-up a whole-blood test based on the interleukin (IL)-4 response to the native Antigen B (AgB) of Echinococcus granulosus. However, AgB is encoded by a multigene family coding for five putative subunits. Therefore, the aims of this study were to analyse the IL-4 response to peptides spanning the immunodominant regions of the five AgB subunits and to evaluate the accuracy of this assay for CE diagnosis. Peptides corresponding to each subunit were combined into five pools. A pool containing all peptides was also used (total pool). IL-4 evaluated by enzyme-linked immunosorbent assay was significantly higher in patients with CE compared to those without (NO-CE subjects) when whole-blood was stimulated with AgB1 and with the total pool. Moreover, IL-4 levels in response to the total pool were significantly increased in patients with active cysts. Receiver Operator Curve analysis identified a cut-off point of 0.59 pg/mL predicting active cysts diagnosis with 71% sensitivity and 82% specificity in serology-positive CE patients. These data, if confirmed in a larger cohort, offer the opportunity to develop new diagnostic tools for CE based on a standardized source of AgB as the peptides.

Original languageEnglish
Article numbere12499
JournalParasite Immunology
Volume39
Issue number12
DOIs
Publication statusPublished - Dec 1 2017

Fingerprint

Echinococcosis
Routine Diagnostic Tests
T-Lymphocytes
Antigens
Interleukin-4
Peptides
Cysts
Immunodominant Epitopes
Immunologic Tests
Hematologic Tests
Serology
Multigene Family
peptide B
Enzyme-Linked Immunosorbent Assay
Sensitivity and Specificity

Keywords

  • cytokine
  • Echinococcus spp
  • ELISA/enzyme-linked immunosorbent assay
  • hydatidosis
  • immunodiagnosis

ASJC Scopus subject areas

  • Parasitology
  • Immunology

Cite this

A T-cell diagnostic test for cystic echinococcosis based on Antigen B peptides. / Petrone, L.; Vanini, V.; Amicosante, M.; Corpolongo, A.; Gomez Morales, M. A.; Ludovisi, A.; Ippolito, G.; Pozio, E.; Teggi, A.; Goletti, D.

In: Parasite Immunology, Vol. 39, No. 12, e12499, 01.12.2017.

Research output: Contribution to journalArticle

Petrone, L. ; Vanini, V. ; Amicosante, M. ; Corpolongo, A. ; Gomez Morales, M. A. ; Ludovisi, A. ; Ippolito, G. ; Pozio, E. ; Teggi, A. ; Goletti, D. / A T-cell diagnostic test for cystic echinococcosis based on Antigen B peptides. In: Parasite Immunology. 2017 ; Vol. 39, No. 12.
@article{f1250c1e33654125b69ec850eeeaa4ca,
title = "A T-cell diagnostic test for cystic echinococcosis based on Antigen B peptides",
abstract = "Cystic echinococcosis (CE) immunodiagnosis is still imperfect. We recently set-up a whole-blood test based on the interleukin (IL)-4 response to the native Antigen B (AgB) of Echinococcus granulosus. However, AgB is encoded by a multigene family coding for five putative subunits. Therefore, the aims of this study were to analyse the IL-4 response to peptides spanning the immunodominant regions of the five AgB subunits and to evaluate the accuracy of this assay for CE diagnosis. Peptides corresponding to each subunit were combined into five pools. A pool containing all peptides was also used (total pool). IL-4 evaluated by enzyme-linked immunosorbent assay was significantly higher in patients with CE compared to those without (NO-CE subjects) when whole-blood was stimulated with AgB1 and with the total pool. Moreover, IL-4 levels in response to the total pool were significantly increased in patients with active cysts. Receiver Operator Curve analysis identified a cut-off point of 0.59 pg/mL predicting active cysts diagnosis with 71{\%} sensitivity and 82{\%} specificity in serology-positive CE patients. These data, if confirmed in a larger cohort, offer the opportunity to develop new diagnostic tools for CE based on a standardized source of AgB as the peptides.",
keywords = "cytokine, Echinococcus spp, ELISA/enzyme-linked immunosorbent assay, hydatidosis, immunodiagnosis",
author = "L. Petrone and V. Vanini and M. Amicosante and A. Corpolongo and {Gomez Morales}, {M. A.} and A. Ludovisi and G. Ippolito and E. Pozio and A. Teggi and D. Goletti",
year = "2017",
month = "12",
day = "1",
doi = "10.1111/pim.12499",
language = "English",
volume = "39",
journal = "Parasite Immunology",
issn = "0141-9838",
publisher = "Wiley-Blackwell",
number = "12",

}

TY - JOUR

T1 - A T-cell diagnostic test for cystic echinococcosis based on Antigen B peptides

AU - Petrone, L.

AU - Vanini, V.

AU - Amicosante, M.

AU - Corpolongo, A.

AU - Gomez Morales, M. A.

AU - Ludovisi, A.

AU - Ippolito, G.

AU - Pozio, E.

AU - Teggi, A.

AU - Goletti, D.

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Cystic echinococcosis (CE) immunodiagnosis is still imperfect. We recently set-up a whole-blood test based on the interleukin (IL)-4 response to the native Antigen B (AgB) of Echinococcus granulosus. However, AgB is encoded by a multigene family coding for five putative subunits. Therefore, the aims of this study were to analyse the IL-4 response to peptides spanning the immunodominant regions of the five AgB subunits and to evaluate the accuracy of this assay for CE diagnosis. Peptides corresponding to each subunit were combined into five pools. A pool containing all peptides was also used (total pool). IL-4 evaluated by enzyme-linked immunosorbent assay was significantly higher in patients with CE compared to those without (NO-CE subjects) when whole-blood was stimulated with AgB1 and with the total pool. Moreover, IL-4 levels in response to the total pool were significantly increased in patients with active cysts. Receiver Operator Curve analysis identified a cut-off point of 0.59 pg/mL predicting active cysts diagnosis with 71% sensitivity and 82% specificity in serology-positive CE patients. These data, if confirmed in a larger cohort, offer the opportunity to develop new diagnostic tools for CE based on a standardized source of AgB as the peptides.

AB - Cystic echinococcosis (CE) immunodiagnosis is still imperfect. We recently set-up a whole-blood test based on the interleukin (IL)-4 response to the native Antigen B (AgB) of Echinococcus granulosus. However, AgB is encoded by a multigene family coding for five putative subunits. Therefore, the aims of this study were to analyse the IL-4 response to peptides spanning the immunodominant regions of the five AgB subunits and to evaluate the accuracy of this assay for CE diagnosis. Peptides corresponding to each subunit were combined into five pools. A pool containing all peptides was also used (total pool). IL-4 evaluated by enzyme-linked immunosorbent assay was significantly higher in patients with CE compared to those without (NO-CE subjects) when whole-blood was stimulated with AgB1 and with the total pool. Moreover, IL-4 levels in response to the total pool were significantly increased in patients with active cysts. Receiver Operator Curve analysis identified a cut-off point of 0.59 pg/mL predicting active cysts diagnosis with 71% sensitivity and 82% specificity in serology-positive CE patients. These data, if confirmed in a larger cohort, offer the opportunity to develop new diagnostic tools for CE based on a standardized source of AgB as the peptides.

KW - cytokine

KW - Echinococcus spp

KW - ELISA/enzyme-linked immunosorbent assay

KW - hydatidosis

KW - immunodiagnosis

UR - http://www.scopus.com/inward/record.url?scp=85034963944&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85034963944&partnerID=8YFLogxK

U2 - 10.1111/pim.12499

DO - 10.1111/pim.12499

M3 - Article

AN - SCOPUS:85034963944

VL - 39

JO - Parasite Immunology

JF - Parasite Immunology

SN - 0141-9838

IS - 12

M1 - e12499

ER -