Abstract
HUVECs (human umbilical vein endothelial cells) are a model for prediction of endothelial dysfunction in various diseases. Cultured cells seem to be a good model for studying molecular processes in endothelial tissue. Nevertheless, they cannot reproduce real physiological status because some biological factors necessarily change in culture conditions. As a consequence, it would be useful to improve preparation of the cellular starting materials from real samples, especially when gene expression studies are further required on the cells. In this work we present a new method for the easy isolation of HUVECs from raw cord blood based on the use of the flow-assisted separative technique, gravitational field-flow fractionation (GrFFF) able to give a relatively pure sample for gene analysis without cell culture steps. The method can be applied to cell populations enzymatically removed from the umbilical vein which can be purified from contaminants cells and used directly for gene expression analysis.
Original language | English |
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Title of host publication | Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 |
Pages | 191-194 |
Number of pages | 4 |
Volume | 3 |
Publication status | Published - 2010 |
Event | Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 - Anaheim, CA, United States Duration: Jun 21 2010 → Jun 24 2010 |
Other
Other | Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 |
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Country/Territory | United States |
City | Anaheim, CA |
Period | 6/21/10 → 6/24/10 |
Keywords
- Cell separation
- Gene expression analysis
- Gravitational field-flow fractionation (GrFFF)
- Human umbilical vein endothelial cells (HUVEC)
- Tag-less cell sorting
ASJC Scopus subject areas
- Bioengineering
- Biomedical Engineering
- Electrical and Electronic Engineering