A tag-less method for the direct isolation of HUVEC (human umbilical vein endothelial cells) by gravitational field-flow fractionation as a starting point for gene expression analysis

D. Lattuada, B. Roda, C. Pignatari, R. Magni, A. Cattaneo, G. Bolis, P. Reschiglian

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

HUVECs (human umbilical vein endothelial cells) are a model for prediction of endothelial dysfunction in various diseases. Cultured cells seem to be a good model for studying molecular processes in endothelial tissue. Nevertheless, they cannot reproduce real physiological status because some biological factors necessarily change in culture conditions. As a consequence, it would be useful to improve preparation of the cellular starting materials from real samples, especially when gene expression studies are further required on the cells. In this work we present a new method for the easy isolation of HUVECs from raw cord blood based on the use of the flow-assisted separative technique, gravitational field-flow fractionation (GrFFF) able to give a relatively pure sample for gene analysis without cell culture steps. The method can be applied to cell populations enzymatically removed from the umbilical vein which can be purified from contaminants cells and used directly for gene expression analysis.

Original languageEnglish
Title of host publicationNanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
Pages191-194
Number of pages4
Volume3
Publication statusPublished - 2010
EventNanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 - Anaheim, CA, United States
Duration: Jun 21 2010Jun 24 2010

Other

OtherNanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
CountryUnited States
CityAnaheim, CA
Period6/21/106/24/10

Keywords

  • Cell separation
  • Gene expression analysis
  • Gravitational field-flow fractionation (GrFFF)
  • Human umbilical vein endothelial cells (HUVEC)
  • Tag-less cell sorting

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering
  • Electrical and Electronic Engineering

Fingerprint Dive into the research topics of 'A tag-less method for the direct isolation of HUVEC (human umbilical vein endothelial cells) by gravitational field-flow fractionation as a starting point for gene expression analysis'. Together they form a unique fingerprint.

  • Cite this

    Lattuada, D., Roda, B., Pignatari, C., Magni, R., Cattaneo, A., Bolis, G., & Reschiglian, P. (2010). A tag-less method for the direct isolation of HUVEC (human umbilical vein endothelial cells) by gravitational field-flow fractionation as a starting point for gene expression analysis. In Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 (Vol. 3, pp. 191-194)