A therapeutic cancer vaccine targeting carcinoembryonic antigen in intestinal carcinomas

Federica Mori, Patrizia Giannetti, Daniela Peruzzi, Domenico Lazzaro, Saverio Giampaoli, Howard L. Kaufman, Gennaro Ciliberto, Nicola La Monica, Luigi Aurisicchio

Research output: Contribution to journalArticle

Abstract

A genetic vaccine platform based on DNA electroporation (DNA-EP) and adenovirus (Ad) was used to generate immune response against human carcinoembryonic antigen (CEA) and antitumor effects in murine models with spontaneous tumors arising in an orthotopic location. CEA transgenic (CEA.Tg) mice treated with the carcinogen 1,2-dimethylhydrazine developed CEA-overexpressing tumors that resembled human sporadic colorectal cancer. APC1638N/CEA hybrid mice, generated by crossing mice carrying the adenomatous polyposis coli (Apc1638N) gene mutation with CEA.Tg mice, are representative of human familial polyposis and develop polyps that overexpress the antigen. In both models, the DNA-EP/Ad vaccine succeeded in breaking immune tolerance and achieved significant antitumor effects in therapeutic settings. Our data suggest that genetic vaccines targeting CEA may be feasible strategies against gut tumors that overexpress the antigen. In addition, these models are powerful systems for evaluating antigen-specific tumor immunity and assessing therapeutic vaccine strategies for human colorectal cancer.

Original languageEnglish
Pages (from-to)125-136
Number of pages12
JournalHuman Gene Therapy
Volume20
Issue number2
DOIs
Publication statusPublished - Feb 1 2009

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ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics

Cite this

Mori, F., Giannetti, P., Peruzzi, D., Lazzaro, D., Giampaoli, S., Kaufman, H. L., Ciliberto, G., La Monica, N., & Aurisicchio, L. (2009). A therapeutic cancer vaccine targeting carcinoembryonic antigen in intestinal carcinomas. Human Gene Therapy, 20(2), 125-136. https://doi.org/10.1089/hum.2008.116