A third polypeptide associated with heavy and light chain subunits of class I HLA antigens in immune interferon-treated human melanoma cells

P. Giacomini, A. Aguzzi, R. Tecce, P. B. Fisher, S. Ferrone

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Recombinant immune interferon (IFN-γ) increases the synthesis, expression and shedding of class I HLA antigens by the cultured human melanoma cell line Colo 38. The magnitude of the IFN-γ-induced changes in class I HLA antigens is dependent on both the dose and the time of exposure to IFN-γ and is more pronounced on the heavy chain subunit than on β2-microglobulin (β2m). In addition, a third, distinct polypeptide with a molecular mass of 14 kDa is present as a major component of the class I HLA molecular pool in IFN-γ-treated melanoma cells. As IFN-γ preferentially increases the synthesis of the heavy chain over that of β2m, the newly induced 14-kDa component appears to quantitatively replace β2m. The stoichiometric relationship between the three molecules suggests that the IFN-γ-induced 14-kDa component may be involved in the insertion of the heavy chain subunit into the plasma membrane.

Original languageEnglish
Pages (from-to)946-951
Number of pages6
JournalEuropean Journal of Immunology
Volume15
Issue number9
DOIs
Publication statusPublished - 1985

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Histocompatibility Antigens Class I
HLA Antigens
Interferons
Interferon-gamma
Melanoma
Light
Peptides
Cell Membrane
Cell Line

ASJC Scopus subject areas

  • Immunology

Cite this

A third polypeptide associated with heavy and light chain subunits of class I HLA antigens in immune interferon-treated human melanoma cells. / Giacomini, P.; Aguzzi, A.; Tecce, R.; Fisher, P. B.; Ferrone, S.

In: European Journal of Immunology, Vol. 15, No. 9, 1985, p. 946-951.

Research output: Contribution to journalArticle

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AU - Ferrone, S.

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