A time-dependent study of nano-mechanical and ultrastructural properties of internal limiting membrane under ocriplasmin treatment

Alberto Mazzini, Francesca Palermo, Valeria Pagliei, Sabrina Romanò, Massimiliano Papi, Giovanna Zimatore, Benedetto Falsini, Stanislao Rizzo, Marco De Spirito, Gabriele Ciasca, Angelo Maria Minnella

Research output: Contribution to journalArticlepeer-review

Abstract

Vitreomacular traction (VMT) syndrome has only been surgically treated for a long time. Recently, enzymatic vitreolysis with ocriplasmin has emerged as a possible option to release VMT and, in some cases, close full thickness macular holes (FTMHs). Despite its clinical relevance, gathering information about the ocriplasmin-induced alterations of the Inner Limiting Membrane (ILM) of the retina in a clinical study is a complex task, mainly because of the inter-individual variability among patients. To obtain more insights into the mechanism underlying the drug action, we studied in-vitro the mechanical and morphological changes of the ILM using Atomic Force Microscopy (AFM). To this aim, we measured the ILM average Young's modulus (YM), hysteresis (H) and adhesion work (A) over time under ocriplasmin treatment. Our data unveil a time-dependent increase in the membrane YM of 19% of its initial value, along with changes in its adhesive and dissipative behavior. Such modifications well correlate with the morphological alterations detected in the AFM imaging mode. Taken all together, the results here presented provide more insights into the mechanism underlying the ocriplasmin action in-vivo, suggesting that it is only able to alter the top-most layer of the vitreal side of the membrane, not compromising the inner ILM structure.

Original languageEnglish
Article number103853
JournalJournal of the Mechanical Behavior of Biomedical Materials
Volume110
DOIs
Publication statusPublished - Oct 2020

Keywords

  • AFM
  • Basal membrane
  • Tissue mechanics

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Mechanics of Materials

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