A toolbox for tuberculosis (TB) diagnosis: An Indian multi-centric study (2006-2008); evaluation of serological assays based on PGL-Tb1 and ESAT-6/CFP10 antigens for TB diagnosis

Philippe H. Lagrange, Satheesh K. Thangaraj, Rajeshwar Dayal, Alaka Deshpande, Nirmal K. Ganguly, Enrico Girardi, Beenu Joshi, Kiran Katoch, Vishwa M. Katoch, Manoj Kumar, Vemu Lakshmi, Marc Leportier, Christophe Longuet, Subbalaxmi V S Malladi, Deepali Mukerjee, Deepthi Nair, Alamelu Raja, Balambal Raman, Camilla Rodrigues, Pratibha SharmaAmit Singh, Sarman Singh, Archana Sodha, Basirudeen Syed Ahamed Kabeer, Guy Vernet, Delia Goletti

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: The aim of this multi-centric prospective study in India was to assess the accuracy of a serological test as an additional tool for diagnosing active tuberculosis (ATB). In particular, an assay based on ELISA using a phenolic glycolipid (PGL-Tb1) or a fusion protein (ESAT-6/CFP10) was compared to the tuberculin skin test (TST) and the microbiological results according to HIV status. Methods: Individuals with and without ATB and HIV infection were enrolled. Serology and TST results were analyzed per se and in combination with the microbiological data. Results: Among the 778 ATB patients, 102 were HIV-infected, 316 HIV-uninfected and 360 had an HIV-unknown status. Of the 945 non-ATB subjects, 559 were at low risk (community adults) and 386 at high risk of M. tuberculosis exposure. Among those with ATB, the sensitivity of ELISA-PGL-Tb1 for ATB was higher than that of ELISA-ESAT-6/CFP10, both in HIV-infected (72.3% versus 63.7%, p = 0.29) and HIV-uninfected/HIV-unknown groups (40.5% versus 28.6%; p

Original languageEnglish
Article numbere96367
JournalPLoS One
Volume9
Issue number5
DOIs
Publication statusPublished - May 5 2014

Fingerprint

Tuberculin
tuberculosis
Assays
Skin
Tuberculosis
HIV
antigens
Antigens
Glycolipids
assays
Tuberculin Test
tuberculin
skin tests
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
Skin Tests
glycolipids
HIV infections
Serologic Tests
immunologic techniques

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

A toolbox for tuberculosis (TB) diagnosis : An Indian multi-centric study (2006-2008); evaluation of serological assays based on PGL-Tb1 and ESAT-6/CFP10 antigens for TB diagnosis. / Lagrange, Philippe H.; Thangaraj, Satheesh K.; Dayal, Rajeshwar; Deshpande, Alaka; Ganguly, Nirmal K.; Girardi, Enrico; Joshi, Beenu; Katoch, Kiran; Katoch, Vishwa M.; Kumar, Manoj; Lakshmi, Vemu; Leportier, Marc; Longuet, Christophe; Malladi, Subbalaxmi V S; Mukerjee, Deepali; Nair, Deepthi; Raja, Alamelu; Raman, Balambal; Rodrigues, Camilla; Sharma, Pratibha; Singh, Amit; Singh, Sarman; Sodha, Archana; Kabeer, Basirudeen Syed Ahamed; Vernet, Guy; Goletti, Delia.

In: PLoS One, Vol. 9, No. 5, e96367, 05.05.2014.

Research output: Contribution to journalArticle

Lagrange, PH, Thangaraj, SK, Dayal, R, Deshpande, A, Ganguly, NK, Girardi, E, Joshi, B, Katoch, K, Katoch, VM, Kumar, M, Lakshmi, V, Leportier, M, Longuet, C, Malladi, SVS, Mukerjee, D, Nair, D, Raja, A, Raman, B, Rodrigues, C, Sharma, P, Singh, A, Singh, S, Sodha, A, Kabeer, BSA, Vernet, G & Goletti, D 2014, 'A toolbox for tuberculosis (TB) diagnosis: An Indian multi-centric study (2006-2008); evaluation of serological assays based on PGL-Tb1 and ESAT-6/CFP10 antigens for TB diagnosis', PLoS One, vol. 9, no. 5, e96367. https://doi.org/10.1371/journal.pone.0096367
Lagrange, Philippe H. ; Thangaraj, Satheesh K. ; Dayal, Rajeshwar ; Deshpande, Alaka ; Ganguly, Nirmal K. ; Girardi, Enrico ; Joshi, Beenu ; Katoch, Kiran ; Katoch, Vishwa M. ; Kumar, Manoj ; Lakshmi, Vemu ; Leportier, Marc ; Longuet, Christophe ; Malladi, Subbalaxmi V S ; Mukerjee, Deepali ; Nair, Deepthi ; Raja, Alamelu ; Raman, Balambal ; Rodrigues, Camilla ; Sharma, Pratibha ; Singh, Amit ; Singh, Sarman ; Sodha, Archana ; Kabeer, Basirudeen Syed Ahamed ; Vernet, Guy ; Goletti, Delia. / A toolbox for tuberculosis (TB) diagnosis : An Indian multi-centric study (2006-2008); evaluation of serological assays based on PGL-Tb1 and ESAT-6/CFP10 antigens for TB diagnosis. In: PLoS One. 2014 ; Vol. 9, No. 5.
@article{c0fed47296d44290ad0250b1a8fca317,
title = "A toolbox for tuberculosis (TB) diagnosis: An Indian multi-centric study (2006-2008); evaluation of serological assays based on PGL-Tb1 and ESAT-6/CFP10 antigens for TB diagnosis",
abstract = "Background: The aim of this multi-centric prospective study in India was to assess the accuracy of a serological test as an additional tool for diagnosing active tuberculosis (ATB). In particular, an assay based on ELISA using a phenolic glycolipid (PGL-Tb1) or a fusion protein (ESAT-6/CFP10) was compared to the tuberculin skin test (TST) and the microbiological results according to HIV status. Methods: Individuals with and without ATB and HIV infection were enrolled. Serology and TST results were analyzed per se and in combination with the microbiological data. Results: Among the 778 ATB patients, 102 were HIV-infected, 316 HIV-uninfected and 360 had an HIV-unknown status. Of the 945 non-ATB subjects, 559 were at low risk (community adults) and 386 at high risk of M. tuberculosis exposure. Among those with ATB, the sensitivity of ELISA-PGL-Tb1 for ATB was higher than that of ELISA-ESAT-6/CFP10, both in HIV-infected (72.3{\%} versus 63.7{\%}, p = 0.29) and HIV-uninfected/HIV-unknown groups (40.5{\%} versus 28.6{\%}; p",
author = "Lagrange, {Philippe H.} and Thangaraj, {Satheesh K.} and Rajeshwar Dayal and Alaka Deshpande and Ganguly, {Nirmal K.} and Enrico Girardi and Beenu Joshi and Kiran Katoch and Katoch, {Vishwa M.} and Manoj Kumar and Vemu Lakshmi and Marc Leportier and Christophe Longuet and Malladi, {Subbalaxmi V S} and Deepali Mukerjee and Deepthi Nair and Alamelu Raja and Balambal Raman and Camilla Rodrigues and Pratibha Sharma and Amit Singh and Sarman Singh and Archana Sodha and Kabeer, {Basirudeen Syed Ahamed} and Guy Vernet and Delia Goletti",
year = "2014",
month = "5",
day = "5",
doi = "10.1371/journal.pone.0096367",
language = "English",
volume = "9",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "5",

}

TY - JOUR

T1 - A toolbox for tuberculosis (TB) diagnosis

T2 - An Indian multi-centric study (2006-2008); evaluation of serological assays based on PGL-Tb1 and ESAT-6/CFP10 antigens for TB diagnosis

AU - Lagrange, Philippe H.

AU - Thangaraj, Satheesh K.

AU - Dayal, Rajeshwar

AU - Deshpande, Alaka

AU - Ganguly, Nirmal K.

AU - Girardi, Enrico

AU - Joshi, Beenu

AU - Katoch, Kiran

AU - Katoch, Vishwa M.

AU - Kumar, Manoj

AU - Lakshmi, Vemu

AU - Leportier, Marc

AU - Longuet, Christophe

AU - Malladi, Subbalaxmi V S

AU - Mukerjee, Deepali

AU - Nair, Deepthi

AU - Raja, Alamelu

AU - Raman, Balambal

AU - Rodrigues, Camilla

AU - Sharma, Pratibha

AU - Singh, Amit

AU - Singh, Sarman

AU - Sodha, Archana

AU - Kabeer, Basirudeen Syed Ahamed

AU - Vernet, Guy

AU - Goletti, Delia

PY - 2014/5/5

Y1 - 2014/5/5

N2 - Background: The aim of this multi-centric prospective study in India was to assess the accuracy of a serological test as an additional tool for diagnosing active tuberculosis (ATB). In particular, an assay based on ELISA using a phenolic glycolipid (PGL-Tb1) or a fusion protein (ESAT-6/CFP10) was compared to the tuberculin skin test (TST) and the microbiological results according to HIV status. Methods: Individuals with and without ATB and HIV infection were enrolled. Serology and TST results were analyzed per se and in combination with the microbiological data. Results: Among the 778 ATB patients, 102 were HIV-infected, 316 HIV-uninfected and 360 had an HIV-unknown status. Of the 945 non-ATB subjects, 559 were at low risk (community adults) and 386 at high risk of M. tuberculosis exposure. Among those with ATB, the sensitivity of ELISA-PGL-Tb1 for ATB was higher than that of ELISA-ESAT-6/CFP10, both in HIV-infected (72.3% versus 63.7%, p = 0.29) and HIV-uninfected/HIV-unknown groups (40.5% versus 28.6%; p

AB - Background: The aim of this multi-centric prospective study in India was to assess the accuracy of a serological test as an additional tool for diagnosing active tuberculosis (ATB). In particular, an assay based on ELISA using a phenolic glycolipid (PGL-Tb1) or a fusion protein (ESAT-6/CFP10) was compared to the tuberculin skin test (TST) and the microbiological results according to HIV status. Methods: Individuals with and without ATB and HIV infection were enrolled. Serology and TST results were analyzed per se and in combination with the microbiological data. Results: Among the 778 ATB patients, 102 were HIV-infected, 316 HIV-uninfected and 360 had an HIV-unknown status. Of the 945 non-ATB subjects, 559 were at low risk (community adults) and 386 at high risk of M. tuberculosis exposure. Among those with ATB, the sensitivity of ELISA-PGL-Tb1 for ATB was higher than that of ELISA-ESAT-6/CFP10, both in HIV-infected (72.3% versus 63.7%, p = 0.29) and HIV-uninfected/HIV-unknown groups (40.5% versus 28.6%; p

UR - http://www.scopus.com/inward/record.url?scp=84900407946&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84900407946&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0096367

DO - 10.1371/journal.pone.0096367

M3 - Article

C2 - 24797271

AN - SCOPUS:84900407946

VL - 9

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 5

M1 - e96367

ER -