A transcription-dependent micrococcal nuclease-resistant fragment of the urokinase-type plasminogen activator promoter interacts with the enhancer

Carmelo Ferrai, Davide Munari, Paolo Luraghi, Lorenza Pecciarini, Maria Giulia Cangi, Claudio Doglioni, Francesco Blasi, Massimo P. Crippa

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We show the interaction between the enhancer and the minimal promoter of urokinase-type plasminogen activator gene during active transcription by coupling micrococcal nuclease digestion of cross-linked, sonicated chromatin, and chromatin immunoprecipitation. This approach allowed the precise identification of the interacting genomic fragments, one of which is resistant to micrococcal nuclease cleavage. The interacting fragments form a single transcriptional control unit, as indicated by their common protein content. Furthermore, we show that the enhancer-MP interaction persists during the early stages of transcription and is lost upon α-amanitin treatment, indicating the requirement for active transcription. Our results support a looping model of interaction between the enhancer and the MP of the urokinase-type plasminogen activator gene.

Original languageEnglish
Pages (from-to)12537-12546
Number of pages10
JournalJournal of Biological Chemistry
Volume282
Issue number17
DOIs
Publication statusPublished - Apr 27 2007

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Micrococcal Nuclease
Urokinase-Type Plasminogen Activator
Transcription
Amanitins
Chromatin
Chromatin Immunoprecipitation
Genes
Digestion
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

A transcription-dependent micrococcal nuclease-resistant fragment of the urokinase-type plasminogen activator promoter interacts with the enhancer. / Ferrai, Carmelo; Munari, Davide; Luraghi, Paolo; Pecciarini, Lorenza; Cangi, Maria Giulia; Doglioni, Claudio; Blasi, Francesco; Crippa, Massimo P.

In: Journal of Biological Chemistry, Vol. 282, No. 17, 27.04.2007, p. 12537-12546.

Research output: Contribution to journalArticle

Ferrai, Carmelo ; Munari, Davide ; Luraghi, Paolo ; Pecciarini, Lorenza ; Cangi, Maria Giulia ; Doglioni, Claudio ; Blasi, Francesco ; Crippa, Massimo P. / A transcription-dependent micrococcal nuclease-resistant fragment of the urokinase-type plasminogen activator promoter interacts with the enhancer. In: Journal of Biological Chemistry. 2007 ; Vol. 282, No. 17. pp. 12537-12546.
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AU - Blasi, Francesco

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