Abstract
We show the interaction between the enhancer and the minimal promoter of urokinase-type plasminogen activator gene during active transcription by coupling micrococcal nuclease digestion of cross-linked, sonicated chromatin, and chromatin immunoprecipitation. This approach allowed the precise identification of the interacting genomic fragments, one of which is resistant to micrococcal nuclease cleavage. The interacting fragments form a single transcriptional control unit, as indicated by their common protein content. Furthermore, we show that the enhancer-MP interaction persists during the early stages of transcription and is lost upon α-amanitin treatment, indicating the requirement for active transcription. Our results support a looping model of interaction between the enhancer and the MP of the urokinase-type plasminogen activator gene.
| Original language | English |
|---|---|
| Pages (from-to) | 12537-12546 |
| Number of pages | 10 |
| Journal | Journal of Biological Chemistry |
| Volume | 282 |
| Issue number | 17 |
| DOIs | |
| Publication status | Published - Apr 27 2007 |
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ASJC Scopus subject areas
- Biochemistry
Cite this
A transcription-dependent micrococcal nuclease-resistant fragment of the urokinase-type plasminogen activator promoter interacts with the enhancer. / Ferrai, Carmelo; Munari, Davide; Luraghi, Paolo; Pecciarini, Lorenza; Cangi, Maria Giulia; Doglioni, Claudio; Blasi, Francesco; Crippa, Massimo P.
In: Journal of Biological Chemistry, Vol. 282, No. 17, 27.04.2007, p. 12537-12546.Research output: Contribution to journal › Article
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TY - JOUR
T1 - A transcription-dependent micrococcal nuclease-resistant fragment of the urokinase-type plasminogen activator promoter interacts with the enhancer
AU - Ferrai, Carmelo
AU - Munari, Davide
AU - Luraghi, Paolo
AU - Pecciarini, Lorenza
AU - Cangi, Maria Giulia
AU - Doglioni, Claudio
AU - Blasi, Francesco
AU - Crippa, Massimo P.
PY - 2007/4/27
Y1 - 2007/4/27
N2 - We show the interaction between the enhancer and the minimal promoter of urokinase-type plasminogen activator gene during active transcription by coupling micrococcal nuclease digestion of cross-linked, sonicated chromatin, and chromatin immunoprecipitation. This approach allowed the precise identification of the interacting genomic fragments, one of which is resistant to micrococcal nuclease cleavage. The interacting fragments form a single transcriptional control unit, as indicated by their common protein content. Furthermore, we show that the enhancer-MP interaction persists during the early stages of transcription and is lost upon α-amanitin treatment, indicating the requirement for active transcription. Our results support a looping model of interaction between the enhancer and the MP of the urokinase-type plasminogen activator gene.
AB - We show the interaction between the enhancer and the minimal promoter of urokinase-type plasminogen activator gene during active transcription by coupling micrococcal nuclease digestion of cross-linked, sonicated chromatin, and chromatin immunoprecipitation. This approach allowed the precise identification of the interacting genomic fragments, one of which is resistant to micrococcal nuclease cleavage. The interacting fragments form a single transcriptional control unit, as indicated by their common protein content. Furthermore, we show that the enhancer-MP interaction persists during the early stages of transcription and is lost upon α-amanitin treatment, indicating the requirement for active transcription. Our results support a looping model of interaction between the enhancer and the MP of the urokinase-type plasminogen activator gene.
UR - http://www.scopus.com/inward/record.url?scp=34250344226&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34250344226&partnerID=8YFLogxK
U2 - 10.1074/jbc.M700867200
DO - 10.1074/jbc.M700867200
M3 - Article
C2 - 17331942
AN - SCOPUS:34250344226
VL - 282
SP - 12537
EP - 12546
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 17
ER -