A transgenic model to analyze the immunoregulatory role of IL-10 secreted by antigen-presenting cells

Hervé Groux, Fraņoise Cottrez, Matthieu Rouleau, Smita Mauze, Svetlana Antonenko, Stephen Hurst, Tom McNeil, Mike Bigler, Maria Grazia Roncarolo, Robert L. Coffman

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Abstract

IL-10 is a cytokine secreted by a wide variety of cells type that has pleiotropic stimulatory and suppressive activities on both lymphoid and myeloid cells in vitro. To analyze the consequences of high IL-10 secretion by APCs in immune responses, we produced transgenic mice expressing human IL- 10 directed by the MHC class II Ea promoter. Despite alterations in the development of T and B cells, no gross abnormalities were detected in peripheral lymphocyte populations or serum Ig levels. However, when immunized using conditions that give either a Th2-type or a Th1-type response, IL-10 transgenic mice failed to mount a significant T or B cell immune response to OVA. IL-10 transgenic mice were also highly susceptible to infection with intracellular pathogens like Listeria monocytogenes or Leishmania major, in contrast to IL-10 transgenic mice, where the transgene was express in T cells. Finally, the recently described stimulatory effect of IL-10 on CD8+ T cells was confirmed by the ability of IL-10 transgenic mice to limit the growth of immunogenic tumors by a CTL-mediated mechanism. These results demonstrate, that, depending on the type of immune response, IL-10 can mediate immunosuppressive or immunostimulatory activities in vivo.

Original languageEnglish
Pages (from-to)1723-1729
Number of pages7
JournalJournal of Immunology
Volume162
Issue number3
Publication statusPublished - Feb 1 1999

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Antigen-Presenting Cells
Interleukin-10
Transgenic Mice
T-Lymphocytes
B-Lymphocytes
Lymphocytes
Leishmania major
Listeria monocytogenes
Myeloid Cells
Immunosuppressive Agents
Transgenes
Cytokines
Growth
Infection
Serum

ASJC Scopus subject areas

  • Immunology

Cite this

Groux, H., Cottrez, F., Rouleau, M., Mauze, S., Antonenko, S., Hurst, S., ... Coffman, R. L. (1999). A transgenic model to analyze the immunoregulatory role of IL-10 secreted by antigen-presenting cells. Journal of Immunology, 162(3), 1723-1729.

A transgenic model to analyze the immunoregulatory role of IL-10 secreted by antigen-presenting cells. / Groux, Hervé; Cottrez, Fraņoise; Rouleau, Matthieu; Mauze, Smita; Antonenko, Svetlana; Hurst, Stephen; McNeil, Tom; Bigler, Mike; Roncarolo, Maria Grazia; Coffman, Robert L.

In: Journal of Immunology, Vol. 162, No. 3, 01.02.1999, p. 1723-1729.

Research output: Contribution to journalArticle

Groux, H, Cottrez, F, Rouleau, M, Mauze, S, Antonenko, S, Hurst, S, McNeil, T, Bigler, M, Roncarolo, MG & Coffman, RL 1999, 'A transgenic model to analyze the immunoregulatory role of IL-10 secreted by antigen-presenting cells', Journal of Immunology, vol. 162, no. 3, pp. 1723-1729.
Groux H, Cottrez F, Rouleau M, Mauze S, Antonenko S, Hurst S et al. A transgenic model to analyze the immunoregulatory role of IL-10 secreted by antigen-presenting cells. Journal of Immunology. 1999 Feb 1;162(3):1723-1729.
Groux, Hervé ; Cottrez, Fraņoise ; Rouleau, Matthieu ; Mauze, Smita ; Antonenko, Svetlana ; Hurst, Stephen ; McNeil, Tom ; Bigler, Mike ; Roncarolo, Maria Grazia ; Coffman, Robert L. / A transgenic model to analyze the immunoregulatory role of IL-10 secreted by antigen-presenting cells. In: Journal of Immunology. 1999 ; Vol. 162, No. 3. pp. 1723-1729.
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