A transgenic mouse model for the detection of cellular stress induced by toxic inorganic compounds

Maria Grazia Sacco, Luigi Zecca, Luca Bagnasco, Giulia Chiesa, Cinzia Parolini, Peter Bromley, Enrica Mira Catò, Romeo Roncucci, Libero A. Clerici, Paolo Vezzoni

Research output: Contribution to journalArticle

Abstract

Transgenic mice for genotoxicity testing have been developed, although no such models have been produced for the evaluation of toxic, nongenotoxic chemical compounds. We have developed a transgenic mouse model for the analysis of toxic inorganic compounds. We engineered a mouse lineage with the human growth hormone (hGH) gene under the control of the human hsp70 promoter, in which a plasma-detectable hGH response can be elicited by exposure to heat shock. In primary cell cultures from these mice, hGH release was observed following treatment with several toxic inorganics. Transgenic mice injected intraperitoneally with sodium arsenite, cadmium chloride, copper sulphate, or methylmercurium chloride showed significant hGH levels in plasma.

Original languageEnglish
Pages (from-to)1392-1397
Number of pages6
JournalNature Biotechnology
Volume15
Issue number13
DOIs
Publication statusPublished - Dec 1997

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Keywords

  • Animal model
  • Heat-shock promoter

ASJC Scopus subject areas

  • Microbiology

Cite this

Sacco, M. G., Zecca, L., Bagnasco, L., Chiesa, G., Parolini, C., Bromley, P., Catò, E. M., Roncucci, R., Clerici, L. A., & Vezzoni, P. (1997). A transgenic mouse model for the detection of cellular stress induced by toxic inorganic compounds. Nature Biotechnology, 15(13), 1392-1397. https://doi.org/10.1038/nbt1297-1392