A TTX-sensitive conductance underlying burst firing in isolated pyramidal neurons from rat neocortex

Ezia Guatteo, Silvana Franceschetti, Alberto Bacci, Giuliano Avanzini, Enzo Wanke

Research output: Contribution to journalArticle

Abstract

Pyramidal neurons were acutely isolated from neocortex slices of 14- to 20-day-old rats and patch-clamped under physiological conditions. Current-clamp recordings revealed firing patterns corresponding to those previously reported in slices as regular spiking (RS) and intrinsically bursting (IB). i.e., single action potentials (AP), trains of regular spikes and bursts with depolarizing after-potentials (DAP). In IB neurons, intracellular perfusion with KF blocked the high-voltage-activated Ca2+ and the Ca2+-dependent K+ currents, revealing APs with a 10-30 ms shoulder at -35 mV (shoulder AP), which was the supporting plateau of the intraburst spikes. The use of the A channel blocker, 4-aminopyridine, caused a three-fold reduction in the AP repolarizing rate. A study of the de- and repolarizing rates modulating the spike shape (sboulder AP, burst or single APs) suggested that the percentage of available A channels could play a crucial role in burst formation. Blockade of the residual T-type Ca2+ current by Ni2+ did not inhibit the AP shoulder, whereas it was completely and reversibly inhibited by 30 nM TTX, which did not affect AP amplitude. The AP rising rate was only halved by 100 nM TTX. The data concerning the A channel-mediated burst formation and the role of the TTX-sensitive conductance have been successfully simulated in a model cell. We suggest that bursting is an intrinsic property of the membrane of neocortex neurons, and is sustained by TTX-sensitive slowly inactivating and/or persistent Na+ conductances.

Original languageEnglish
Pages (from-to)1-12
Number of pages12
JournalBrain Research
Volume741
Issue number1-2
DOIs
Publication statusPublished - Nov 25 1996

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Pyramidal Cells
Neocortex
Action Potentials
Neurons
4-Aminopyridine
Perfusion
Membranes

Keywords

  • burst firing
  • pyramidal neuron
  • sensorimotor cortex

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

A TTX-sensitive conductance underlying burst firing in isolated pyramidal neurons from rat neocortex. / Guatteo, Ezia; Franceschetti, Silvana; Bacci, Alberto; Avanzini, Giuliano; Wanke, Enzo.

In: Brain Research, Vol. 741, No. 1-2, 25.11.1996, p. 1-12.

Research output: Contribution to journalArticle

Guatteo, Ezia ; Franceschetti, Silvana ; Bacci, Alberto ; Avanzini, Giuliano ; Wanke, Enzo. / A TTX-sensitive conductance underlying burst firing in isolated pyramidal neurons from rat neocortex. In: Brain Research. 1996 ; Vol. 741, No. 1-2. pp. 1-12.
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