A tumor-associated fibronectin isoform generated by alternative splicing of messenger RNA precursors

B. Carnemolla, E. Balza, A. Siri, L. Zardi, M. R. Nicotra, A. Bigotti, P. G. Natali

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Abstract

Fibronectin (FN) represents the mixture of a number of structurally different molecules (isoforms) whose make-up varies depending on the FN sources. FN from cultured transformed human cells has a very different isoform composition with respect to its normal counterpart. In fact, SV-40-transformed WI-38VAI3 human fibroblasts produce high levels of a FN isoform (B-FN) which is very poorly expressed in their nomal, WI-38, counterpart. We have recently demonstrated that the B-FN isoform derives from a differential splicing pattern of the FN primary transcript which leads, in transformed cells, to a high level expression of the exon ED-B (Zardi, L., B. Carnemolla, A. Siri, T.E. Petersen, G. Paolella, G. Sebastio, and F.E. Baralle. 1987. EMBO (Eur. Mol. Biol. Organ.) J. 6:2337-2342). Here we report on the production and characterization of a monoclonal antibody (BC-1) which recognizes an epitope within the protein sequence coded for by the ED-B exon. This monoclonal antibody makes it possible to carry out immunohistochemical analysis of the distribution of the ED-B-containing FN isoform (B-FN) in human tissues. The results show that while in normal, adult, human tissues total FN has a widespread distribution, the B-FN isoform is restricted only to synovial cells, to some vessels and areas of the interstitium of the ovary, and to the myometrium. On the contrary, the B-FN isoform has a much greater expression in fetal and tumor tissues. These results demonstrate that, in vivo, different FN isoforms have a differential distribution and indicate that the B-FN isoform may play a role in ontogenesis and oncogenetic processes.

Original languageEnglish
Pages (from-to)1139-1148
Number of pages10
JournalJournal of Cell Biology
Volume108
Issue number3
Publication statusPublished - 1989

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ASJC Scopus subject areas

  • Cell Biology

Cite this

Carnemolla, B., Balza, E., Siri, A., Zardi, L., Nicotra, M. R., Bigotti, A., & Natali, P. G. (1989). A tumor-associated fibronectin isoform generated by alternative splicing of messenger RNA precursors. Journal of Cell Biology, 108(3), 1139-1148.