A Two-Step Protocol to Erase Human Skin Fibroblasts and Convert Them into Trophoblast-like Cells

Sharon Arcuri, Fulvio Gandolfi, Edgardo Somigliana, Tiziana A.L. Brevini

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The first differentiation event in mammalian embryos is the formation of the trophectoderm, which is the progenitor of the outer epithelial component of the placenta and supports the fetus during intrauterine life. Our understanding of these events is limited, particularly in human, because of ethical and legal restrictions and availability of adequate in vitro models would be very advantageous. Here we describe a method that converts human fibroblasts into trophoblast-like cells, combining the use of 5-azacytidine-CR (5-aza-CR) to erase the original cell phenotype and a cocktail containing bone morphogenetic protein 4 (BMP4) with inhibitors of the Activin/Nodal/ERK signaling pathways, to drive erased fibroblasts into the trophoblastic differentiation. This innovative method uses very easily accessible cells to derive trophoblast-like cells and it can be useful to study embryo implantation disorders related to aging.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages151-158
Number of pages8
DOIs
Publication statusPublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2273
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • 5-aza-CR
  • Activin/Nodal/ERK inhibitors
  • BMP4
  • Epigenetics
  • Fibroblast
  • Trophoblast

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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