A validated method for urinary cotinine quantification used to classify active and environmental tobacco smoke exposure

Silvia Fustinoni, Laura Campo, Elisa Polledri, Rosa Mercadante, Laura Erspamer, Andrea Ranzi, Paolo Lauriola, Carlo Alberto Goldoni, PierAlberto A. Bertazzi

Research output: Contribution to journalArticlepeer-review

Abstract

The aims of this research were to develop and validate a simple, sensitive method to determine un-conjugated urinary cotinine (COT-U) levels and to investigate its ability to discriminate active and environmental tobacco smoke (ETS) exposure. For this assay, urine was diluted with water, cotinine-d3 was added as an internal standard, and the sample was separated by a C18 column and analysed by triple quadrupole mass spectrometry. The quantification limit was 0.1 μg/L, range of linearity was 0.1-4000 μg/L, intra- and inter-run precision were

Original languageEnglish
Pages (from-to)447-456
Number of pages10
JournalCurrent Analytical Chemistry
Volume9
Issue number3
DOIs
Publication statusPublished - 2013

Keywords

  • Active tobacco smoke
  • Biomonitoring
  • Environmental tobacco smoke
  • LC-MS/MS
  • Urinary cotinine

ASJC Scopus subject areas

  • Analytical Chemistry

Fingerprint Dive into the research topics of 'A validated method for urinary cotinine quantification used to classify active and environmental tobacco smoke exposure'. Together they form a unique fingerprint.

Cite this