A variation in a Pit-1 site in the growth hormone gene (GH1) promoter induces a differential transcriptional activity

Mara Giordano, Michela Godi, Francesca Giacopelli, Monica Lessi, Simona Mellone, Roberta Paracchini, Antonella Petri, Jaele Bellone, Roberto Ravazzolo, Gianni Bona, Patricia Momigliano-Richiardi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The proximal promoter of the human growth hormone gene (GH1) is highly polymorphic. We tested if promoter haplotypes differing at possibly functional sites, namely -278T/G (in the NF1 binding site), -75A/G (in the proximal Pit-1 binding site) and -57G/T (in the VDR binding site), induced a different luciferase activity when transfected in a rat pituitary cell line. The presence of a G instead of an A at position -75 induced a more than two-fold reduced activity (p <0.0001). In accordance with this findings the electrophoretic mobility shift assay demonstrated a reduced affinity of the -75G for the pituitary transcription factor Pit-1. Despite the strong effect of this polymorphism in vitro, the -75G variation was not associated to an impairment of the GH secretion in vivo.

Original languageEnglish
Pages (from-to)51-57
Number of pages7
JournalMolecular and Cellular Endocrinology
Volume249
Issue number1-2
DOIs
Publication statusPublished - Apr 25 2006

Fingerprint

Growth Hormone
Genes
Binding Sites
Transcription Factor Pit-1
Electrophoretic mobility
Human Growth Hormone
Electrophoretic Mobility Shift Assay
Polymorphism
Luciferases
Haplotypes
Rats
Assays
Cells
Cell Line
In Vitro Techniques

Keywords

  • Growth hormone gene
  • Luciferase assay
  • Pit-1
  • Polymorphism

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Giordano, M., Godi, M., Giacopelli, F., Lessi, M., Mellone, S., Paracchini, R., ... Momigliano-Richiardi, P. (2006). A variation in a Pit-1 site in the growth hormone gene (GH1) promoter induces a differential transcriptional activity. Molecular and Cellular Endocrinology, 249(1-2), 51-57. https://doi.org/10.1016/j.mce.2006.01.006

A variation in a Pit-1 site in the growth hormone gene (GH1) promoter induces a differential transcriptional activity. / Giordano, Mara; Godi, Michela; Giacopelli, Francesca; Lessi, Monica; Mellone, Simona; Paracchini, Roberta; Petri, Antonella; Bellone, Jaele; Ravazzolo, Roberto; Bona, Gianni; Momigliano-Richiardi, Patricia.

In: Molecular and Cellular Endocrinology, Vol. 249, No. 1-2, 25.04.2006, p. 51-57.

Research output: Contribution to journalArticle

Giordano, M, Godi, M, Giacopelli, F, Lessi, M, Mellone, S, Paracchini, R, Petri, A, Bellone, J, Ravazzolo, R, Bona, G & Momigliano-Richiardi, P 2006, 'A variation in a Pit-1 site in the growth hormone gene (GH1) promoter induces a differential transcriptional activity', Molecular and Cellular Endocrinology, vol. 249, no. 1-2, pp. 51-57. https://doi.org/10.1016/j.mce.2006.01.006
Giordano, Mara ; Godi, Michela ; Giacopelli, Francesca ; Lessi, Monica ; Mellone, Simona ; Paracchini, Roberta ; Petri, Antonella ; Bellone, Jaele ; Ravazzolo, Roberto ; Bona, Gianni ; Momigliano-Richiardi, Patricia. / A variation in a Pit-1 site in the growth hormone gene (GH1) promoter induces a differential transcriptional activity. In: Molecular and Cellular Endocrinology. 2006 ; Vol. 249, No. 1-2. pp. 51-57.
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