A versatile tool for stable inhibition of microRNA Activity

Paride Pelucchi, Valeria Tria, Valentina Martino, Davood Sabour, Giovanni Bertalot, Stefano Molgora, Mira Palizban, Martin Götte, Ileana Zucchi, Rolland A. Reinbold

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

MicroRNAs (miRNAs are a class of small RNAs (18-22 nt that post transcriptionally regulate gene expression by binding to complementary sequences on target mRNAs, resulting in translational repression or target degradation and gene silencing. As aberrant expression of miRNAs is implicated in important diseases including cancer miRNA-based therapies are under intensive investigation. We optimized strategies to stably or conditionally generate miRNA inhibitors for a continuous block of miRNA activity that allows for probing miRNA function in long-term cell culture experiments, cancer xenografts, 3D tissue models and for in vivo studies with transgenic organisms.

Original languageEnglish
Pages (from-to)861-871
Number of pages11
JournalBiology
Volume2
Issue number3
DOIs
Publication statusPublished - Jun 28 2013

Fingerprint

MicroRNAs
microRNA
neoplasms
gene silencing
in vivo studies
Genetically Modified Organisms
cell culture
Gene Silencing
genetically modified organisms
RNA
Cell culture
Heterografts
Gene expression
gene expression
therapeutics
Neoplasms
degradation
organisms
Cell Culture Techniques
Genes

Keywords

  • Lentivirus
  • miR-inhibitor
  • miRNA

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Pelucchi, P., Tria, V., Martino, V., Sabour, D., Bertalot, G., Molgora, S., ... Reinbold, R. A. (2013). A versatile tool for stable inhibition of microRNA Activity. Biology, 2(3), 861-871. https://doi.org/10.3390/biology2030861

A versatile tool for stable inhibition of microRNA Activity. / Pelucchi, Paride; Tria, Valeria; Martino, Valentina; Sabour, Davood; Bertalot, Giovanni; Molgora, Stefano; Palizban, Mira; Götte, Martin; Zucchi, Ileana; Reinbold, Rolland A.

In: Biology, Vol. 2, No. 3, 28.06.2013, p. 861-871.

Research output: Contribution to journalArticle

Pelucchi, P, Tria, V, Martino, V, Sabour, D, Bertalot, G, Molgora, S, Palizban, M, Götte, M, Zucchi, I & Reinbold, RA 2013, 'A versatile tool for stable inhibition of microRNA Activity', Biology, vol. 2, no. 3, pp. 861-871. https://doi.org/10.3390/biology2030861
Pelucchi P, Tria V, Martino V, Sabour D, Bertalot G, Molgora S et al. A versatile tool for stable inhibition of microRNA Activity. Biology. 2013 Jun 28;2(3):861-871. https://doi.org/10.3390/biology2030861
Pelucchi, Paride ; Tria, Valeria ; Martino, Valentina ; Sabour, Davood ; Bertalot, Giovanni ; Molgora, Stefano ; Palizban, Mira ; Götte, Martin ; Zucchi, Ileana ; Reinbold, Rolland A. / A versatile tool for stable inhibition of microRNA Activity. In: Biology. 2013 ; Vol. 2, No. 3. pp. 861-871.
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