A versatile tool for stable inhibition of microRNA Activity

Paride Pelucchi, Valeria Tria, Valentina Martino, Davood Sabour, Giovanni Bertalot, Stefano Molgora, Mira Palizban, Martin Götte, Ileana Zucchi, Rolland A. Reinbold

Research output: Contribution to journalArticle

Abstract

MicroRNAs (miRNAs are a class of small RNAs (18-22 nt that post transcriptionally regulate gene expression by binding to complementary sequences on target mRNAs, resulting in translational repression or target degradation and gene silencing. As aberrant expression of miRNAs is implicated in important diseases including cancer miRNA-based therapies are under intensive investigation. We optimized strategies to stably or conditionally generate miRNA inhibitors for a continuous block of miRNA activity that allows for probing miRNA function in long-term cell culture experiments, cancer xenografts, 3D tissue models and for in vivo studies with transgenic organisms.

Original languageEnglish
Pages (from-to)861-871
Number of pages11
JournalBiology
Volume2
Issue number3
DOIs
Publication statusPublished - Jun 28 2013

Keywords

  • Lentivirus
  • miR-inhibitor
  • miRNA

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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  • Cite this

    Pelucchi, P., Tria, V., Martino, V., Sabour, D., Bertalot, G., Molgora, S., Palizban, M., Götte, M., Zucchi, I., & Reinbold, R. A. (2013). A versatile tool for stable inhibition of microRNA Activity. Biology, 2(3), 861-871. https://doi.org/10.3390/biology2030861