A "weighted" fluorescence in situ hybridization strengthens the favorable prognostic value of 1p/19q codeletion in pure and mixed oligodendroglial tumors

Rebecca Senetta, Ludovica Verdun Di Cantogno, Luigi Chiusa, Isabella Castellano, Patrizia Gugliotta, Anna Sapino, Paola Cassoni

Research output: Contribution to journalArticle

Abstract

Evaluation of the molecular status of 1p and 19q is a major relevant diagnostic, prognostic, and predictive tool for oligodendroglial brain tumors. Fluorescence in situ hybridization (FISH) is the most commonly used technique for determining 1p and 19q allelic losses, but it lacks fully standardized criteria for analysis. This lack of standardization has led to interinstitutional disagreement in the interpretation of results, thereby contributing to a "gray prognostic zone" that includes codeleted patients with an unexpectedly unfavorable outcome. To optimize the prognostic potential of 1p/19q status determination, we first compared the actual criteria used for FISH reading (i.e. different ratio cutoff values and the percentage of neoplastic nuclei carrying this chromosomal deletion) in a retrospective series of 143 pure and mixed oligodendroglial tumors. We then created a "weighted" FISH reading based on the merged ratio and percentage of neoplastic cells carrying the deletion that was further differentially modulated for 1p and 19q, respectively. This weighted codeletion setting significantly strengthened the favorable prognostic power of 1p/19q losses by reducing the number of poor outcomes from 42% to 12.5% for patients with codeleted tumors. Thus, by identifying as codeleted only those cases with more than 50% of cells having a combined loss of 1p (using 0.7 ratio cutoff) and 19q (using 0.8 ratio cutoff) arms, we created a molecular report that bears higher clinical impact and strengthens the prognostic potential of 1p/19q allelic loss.

Original languageEnglish
Pages (from-to)432-441
Number of pages10
JournalJournal of Neuropathology and Experimental Neurology
Volume72
Issue number5
DOIs
Publication statusPublished - May 2013

Fingerprint

Fluorescence In Situ Hybridization
Loss of Heterozygosity
Reading
Neoplasms
Brain Neoplasms

Keywords

  • 1p/19q status
  • Fluorescence in situ hybridization analysis
  • Glioma
  • Oligodendroglial tumors
  • Prognostic factor

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Clinical Neurology
  • Neurology
  • Cellular and Molecular Neuroscience

Cite this

A "weighted" fluorescence in situ hybridization strengthens the favorable prognostic value of 1p/19q codeletion in pure and mixed oligodendroglial tumors. / Senetta, Rebecca; Verdun Di Cantogno, Ludovica; Chiusa, Luigi; Castellano, Isabella; Gugliotta, Patrizia; Sapino, Anna; Cassoni, Paola.

In: Journal of Neuropathology and Experimental Neurology, Vol. 72, No. 5, 05.2013, p. 432-441.

Research output: Contribution to journalArticle

Senetta, Rebecca ; Verdun Di Cantogno, Ludovica ; Chiusa, Luigi ; Castellano, Isabella ; Gugliotta, Patrizia ; Sapino, Anna ; Cassoni, Paola. / A "weighted" fluorescence in situ hybridization strengthens the favorable prognostic value of 1p/19q codeletion in pure and mixed oligodendroglial tumors. In: Journal of Neuropathology and Experimental Neurology. 2013 ; Vol. 72, No. 5. pp. 432-441.
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