A WT1 Expressing Metastatic Human Kaposi Sarcoma Xenograft Model

Erzsébet Rásó; Lívia Mészáros; Adriana Albini; József Tímár

A WT1 Expressing Metastatic Human Kaposi Sarcoma Xenograft Model

Erzsébet Rásó, Lívia Mészáros, Adriana Albini, József Tímár

Arcispedale Santa Maria Nuova

Research output: Contribution to journal › Article › peer-review

Abstract

We have established a non-metastatic and a metastatic human Kaposi sarcoma (KS) xenograft model in SCID mice by injecting KS-Imm cells subcutaneously and intrasplenically, respectively. KS-Imm cells expressed endothelial markers, CD34 and vWF in vivo. Futhermore, we have shown that these cells express all the splice variants of the WT1 gene and WT1^wt protein in vitro and in vivo detected by nested PCR and immunohistochemistry. WT1 expression in the peripheral blood was only detectable in case of metastatic KS model suggesting it as a molecular marker of progression.

Original language	English
Pages (from-to)	22-25
Number of pages	4
Journal	Pathology and Oncology Research
Volume	10
Issue number	1
Publication status	Published - 2004

Keywords

Kaposi sarcoma
Metastasis
WT1 expression
Xenograft model

ASJC Scopus subject areas

Cancer Research
Oncology
Pathology and Forensic Medicine

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abstract = "We have established a non-metastatic and a metastatic human Kaposi sarcoma (KS) xenograft model in SCID mice by injecting KS-Imm cells subcutaneously and intrasplenically, respectively. KS-Imm cells expressed endothelial markers, CD34 and vWF in vivo. Futhermore, we have shown that these cells express all the splice variants of the WT1 gene and WT1wt protein in vitro and in vivo detected by nested PCR and immunohistochemistry. WT1 expression in the peripheral blood was only detectable in case of metastatic KS model suggesting it as a molecular marker of progression.",

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AU - Mészáros, Lívia

AU - Albini, Adriana

AU - Tímár, József

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N2 - We have established a non-metastatic and a metastatic human Kaposi sarcoma (KS) xenograft model in SCID mice by injecting KS-Imm cells subcutaneously and intrasplenically, respectively. KS-Imm cells expressed endothelial markers, CD34 and vWF in vivo. Futhermore, we have shown that these cells express all the splice variants of the WT1 gene and WT1wt protein in vitro and in vivo detected by nested PCR and immunohistochemistry. WT1 expression in the peripheral blood was only detectable in case of metastatic KS model suggesting it as a molecular marker of progression.

AB - We have established a non-metastatic and a metastatic human Kaposi sarcoma (KS) xenograft model in SCID mice by injecting KS-Imm cells subcutaneously and intrasplenically, respectively. KS-Imm cells expressed endothelial markers, CD34 and vWF in vivo. Futhermore, we have shown that these cells express all the splice variants of the WT1 gene and WT1wt protein in vitro and in vivo detected by nested PCR and immunohistochemistry. WT1 expression in the peripheral blood was only detectable in case of metastatic KS model suggesting it as a molecular marker of progression.

KW - Kaposi sarcoma

KW - Metastasis

KW - WT1 expression

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