Ab interno trabeculectomy: Ultrastructural evidence and early tissue response in a human eye

Ettore Ferrari, Fulvia Ortolani, Lucia Petrelli, Magali Contin, Derri Roman Pognuz, Maurizio Marchini, Francesco Bandello

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Purpose: To report the results of ultrastructural analysis of the postoperative effects of ab interno trabeculectomy in a human eye. Setting: Department of Ophthalmology, Palmanova Hospital, Palmanova, Udine, Italy. Methods: A 60-year-old woman with cataract and glaucoma had enucleation for a choroidal melanoma 10 days after ab interno trabeculectomy combined with phacoemulsification. A second ab interno trabeculectomy was performed after enucleation to evaluate the outcomes of the previous trabeculectomy. Light and transmission electron microscopy analyses were performed on samples excised from areas (1) not subjected to a procedure (control samples), (2) that had ab interno trabeculectomy before enucleation, and (3) that had ab interno trabeculectomy immediately after enucleation. Results: Control samples showed normal trabecular features. Semithin sections of all ab interno trabeculectomy samples showed full-thickness removal of trabeculum segments, with Schlemm's canal lumen opening into the anterior chamber and apparent preservation of the adjacent structures. On ultrathin sections of samples that had ab interno trabeculectomy before enucleation, the endothelium lining the outer wall of Schlemm's canal and other angle components showed intact ultrastructural features. In trabecular beams that were not removed, the extracellular matrix appeared to have maintained its fine texture and was free of activated fibroblasts or leucocyte infiltrates. Conclusions: Observations confirm that ab interno trabeculectomy causes direct communication between Schlemm's canal lumen and the anterior chamber in vivo and immediately after enucleation during the early postoperative period. The absence of an evident inflammatory reaction in the examined case should be considered with caution because of possible tumor-induced immune suppression.

Original languageEnglish
Pages (from-to)1750-1753
Number of pages4
JournalJournal of Cataract and Refractive Surgery
Volume33
Issue number10
DOIs
Publication statusPublished - Oct 2007

Fingerprint

Trabeculectomy
Anterior Chamber
Phacoemulsification
Ophthalmology
Transmission Electron Microscopy
Postoperative Period
Glaucoma
Cataract
Italy
Endothelium
Extracellular Matrix
Melanoma
Leukocytes
Fibroblasts
Communication
Light

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Ab interno trabeculectomy : Ultrastructural evidence and early tissue response in a human eye. / Ferrari, Ettore; Ortolani, Fulvia; Petrelli, Lucia; Contin, Magali; Pognuz, Derri Roman; Marchini, Maurizio; Bandello, Francesco.

In: Journal of Cataract and Refractive Surgery, Vol. 33, No. 10, 10.2007, p. 1750-1753.

Research output: Contribution to journalArticle

Ferrari, Ettore ; Ortolani, Fulvia ; Petrelli, Lucia ; Contin, Magali ; Pognuz, Derri Roman ; Marchini, Maurizio ; Bandello, Francesco. / Ab interno trabeculectomy : Ultrastructural evidence and early tissue response in a human eye. In: Journal of Cataract and Refractive Surgery. 2007 ; Vol. 33, No. 10. pp. 1750-1753.
@article{db48290fb2814a99b607164123856b5d,
title = "Ab interno trabeculectomy: Ultrastructural evidence and early tissue response in a human eye",
abstract = "Purpose: To report the results of ultrastructural analysis of the postoperative effects of ab interno trabeculectomy in a human eye. Setting: Department of Ophthalmology, Palmanova Hospital, Palmanova, Udine, Italy. Methods: A 60-year-old woman with cataract and glaucoma had enucleation for a choroidal melanoma 10 days after ab interno trabeculectomy combined with phacoemulsification. A second ab interno trabeculectomy was performed after enucleation to evaluate the outcomes of the previous trabeculectomy. Light and transmission electron microscopy analyses were performed on samples excised from areas (1) not subjected to a procedure (control samples), (2) that had ab interno trabeculectomy before enucleation, and (3) that had ab interno trabeculectomy immediately after enucleation. Results: Control samples showed normal trabecular features. Semithin sections of all ab interno trabeculectomy samples showed full-thickness removal of trabeculum segments, with Schlemm's canal lumen opening into the anterior chamber and apparent preservation of the adjacent structures. On ultrathin sections of samples that had ab interno trabeculectomy before enucleation, the endothelium lining the outer wall of Schlemm's canal and other angle components showed intact ultrastructural features. In trabecular beams that were not removed, the extracellular matrix appeared to have maintained its fine texture and was free of activated fibroblasts or leucocyte infiltrates. Conclusions: Observations confirm that ab interno trabeculectomy causes direct communication between Schlemm's canal lumen and the anterior chamber in vivo and immediately after enucleation during the early postoperative period. The absence of an evident inflammatory reaction in the examined case should be considered with caution because of possible tumor-induced immune suppression.",
author = "Ettore Ferrari and Fulvia Ortolani and Lucia Petrelli and Magali Contin and Pognuz, {Derri Roman} and Maurizio Marchini and Francesco Bandello",
year = "2007",
month = "10",
doi = "10.1016/j.jcrs.2007.05.035",
language = "English",
volume = "33",
pages = "1750--1753",
journal = "Journal of Cataract and Refractive Surgery",
issn = "0886-3350",
publisher = "Elsevier Inc.",
number = "10",

}

TY - JOUR

T1 - Ab interno trabeculectomy

T2 - Ultrastructural evidence and early tissue response in a human eye

AU - Ferrari, Ettore

AU - Ortolani, Fulvia

AU - Petrelli, Lucia

AU - Contin, Magali

AU - Pognuz, Derri Roman

AU - Marchini, Maurizio

AU - Bandello, Francesco

PY - 2007/10

Y1 - 2007/10

N2 - Purpose: To report the results of ultrastructural analysis of the postoperative effects of ab interno trabeculectomy in a human eye. Setting: Department of Ophthalmology, Palmanova Hospital, Palmanova, Udine, Italy. Methods: A 60-year-old woman with cataract and glaucoma had enucleation for a choroidal melanoma 10 days after ab interno trabeculectomy combined with phacoemulsification. A second ab interno trabeculectomy was performed after enucleation to evaluate the outcomes of the previous trabeculectomy. Light and transmission electron microscopy analyses were performed on samples excised from areas (1) not subjected to a procedure (control samples), (2) that had ab interno trabeculectomy before enucleation, and (3) that had ab interno trabeculectomy immediately after enucleation. Results: Control samples showed normal trabecular features. Semithin sections of all ab interno trabeculectomy samples showed full-thickness removal of trabeculum segments, with Schlemm's canal lumen opening into the anterior chamber and apparent preservation of the adjacent structures. On ultrathin sections of samples that had ab interno trabeculectomy before enucleation, the endothelium lining the outer wall of Schlemm's canal and other angle components showed intact ultrastructural features. In trabecular beams that were not removed, the extracellular matrix appeared to have maintained its fine texture and was free of activated fibroblasts or leucocyte infiltrates. Conclusions: Observations confirm that ab interno trabeculectomy causes direct communication between Schlemm's canal lumen and the anterior chamber in vivo and immediately after enucleation during the early postoperative period. The absence of an evident inflammatory reaction in the examined case should be considered with caution because of possible tumor-induced immune suppression.

AB - Purpose: To report the results of ultrastructural analysis of the postoperative effects of ab interno trabeculectomy in a human eye. Setting: Department of Ophthalmology, Palmanova Hospital, Palmanova, Udine, Italy. Methods: A 60-year-old woman with cataract and glaucoma had enucleation for a choroidal melanoma 10 days after ab interno trabeculectomy combined with phacoemulsification. A second ab interno trabeculectomy was performed after enucleation to evaluate the outcomes of the previous trabeculectomy. Light and transmission electron microscopy analyses were performed on samples excised from areas (1) not subjected to a procedure (control samples), (2) that had ab interno trabeculectomy before enucleation, and (3) that had ab interno trabeculectomy immediately after enucleation. Results: Control samples showed normal trabecular features. Semithin sections of all ab interno trabeculectomy samples showed full-thickness removal of trabeculum segments, with Schlemm's canal lumen opening into the anterior chamber and apparent preservation of the adjacent structures. On ultrathin sections of samples that had ab interno trabeculectomy before enucleation, the endothelium lining the outer wall of Schlemm's canal and other angle components showed intact ultrastructural features. In trabecular beams that were not removed, the extracellular matrix appeared to have maintained its fine texture and was free of activated fibroblasts or leucocyte infiltrates. Conclusions: Observations confirm that ab interno trabeculectomy causes direct communication between Schlemm's canal lumen and the anterior chamber in vivo and immediately after enucleation during the early postoperative period. The absence of an evident inflammatory reaction in the examined case should be considered with caution because of possible tumor-induced immune suppression.

UR - http://www.scopus.com/inward/record.url?scp=34548771172&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548771172&partnerID=8YFLogxK

U2 - 10.1016/j.jcrs.2007.05.035

DO - 10.1016/j.jcrs.2007.05.035

M3 - Article

C2 - 17889771

AN - SCOPUS:34548771172

VL - 33

SP - 1750

EP - 1753

JO - Journal of Cataract and Refractive Surgery

JF - Journal of Cataract and Refractive Surgery

SN - 0886-3350

IS - 10

ER -