TY - JOUR
T1 - ABC protein transport of MRI contrast agents in canalicular rat liver plasma vesicles and yeast vacuoles
AU - Pascolo, L.
AU - Petrovic, S.
AU - Cupelli, F.
AU - Bruschi, C. V.
AU - Anelli, P. L.
AU - Lorusso, V.
AU - Visigalli, M.
AU - Uggeri, F.
AU - Tiribelli, C.
PY - 2001
Y1 - 2001
N2 - The mechanism of excretion into bile of hepatospecific magnetic resonance imaging (MRI) contrast media employed labeled Gd-reagents EOB.DTPA, BOPTA, B 20790 (iopanoate-linked), and B 21690 (glycocholate-linked) for measurement in rat liver canalicular plasma membrane vesicles and yeast vacuoles. The presence of ATP gave threefold greater transport of B 20790 and B 21690 than of EOB.DTPA and BOPTA. In yeast vacuoles the ATP stimulatory effect was eightfold with B 20790 and fivefold greater for B 21690, whereas in YCF1- or YLLO115w-deleted yeast cells the transport was significantly reduced and absent from double mutants, YCF1 and YLLO15w. The transport was similar in wild-type and deletant cells for B 21690; taurocholate gave 85% inhibition. These data suggest that bilary secretion of structurally related MRI agents depend on molecular structure. The findings are suggestive as of possible value for clinical diagnosis of inherited hyperbilirubinemias and other liver disorders.
AB - The mechanism of excretion into bile of hepatospecific magnetic resonance imaging (MRI) contrast media employed labeled Gd-reagents EOB.DTPA, BOPTA, B 20790 (iopanoate-linked), and B 21690 (glycocholate-linked) for measurement in rat liver canalicular plasma membrane vesicles and yeast vacuoles. The presence of ATP gave threefold greater transport of B 20790 and B 21690 than of EOB.DTPA and BOPTA. In yeast vacuoles the ATP stimulatory effect was eightfold with B 20790 and fivefold greater for B 21690, whereas in YCF1- or YLLO115w-deleted yeast cells the transport was significantly reduced and absent from double mutants, YCF1 and YLLO15w. The transport was similar in wild-type and deletant cells for B 21690; taurocholate gave 85% inhibition. These data suggest that bilary secretion of structurally related MRI agents depend on molecular structure. The findings are suggestive as of possible value for clinical diagnosis of inherited hyperbilirubinemias and other liver disorders.
UR - http://www.scopus.com/inward/record.url?scp=0034815177&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034815177&partnerID=8YFLogxK
U2 - 10.1006/bbrc.2001.4318
DO - 10.1006/bbrc.2001.4318
M3 - Article
C2 - 11263971
AN - SCOPUS:0034815177
VL - 282
SP - 60
EP - 66
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -