ABCB1 gene polymorphisms and expression of P-glycoprotein and long-term prognosis in colorectal cancer

Sara De Iudicibus, Alessandro De Pellegrin, Gabriele Stocco, Fiora Bartoli, Rossana Bussani, Giuliana Decorti

Research output: Contribution to journalArticlepeer-review

Abstract

Background: P-glycoprotein (Pgp), encoded by the ATP-binding cassette B1 (ABCB1) gene, is an efflux transporter located on the luminal side of intestinal epithelial cells, which protects the gut from endogenous and exogenous toxins. The association of two ABCB1 polymorphisms with the occurrence of colon cancer and long-term prognosis was evaluated in a selected patient population. The expression of Pgp in neoplastic and normal intestinal mucosa was also studied. Patients and Methods: Archival material from 51 patients, in Dukes stage B2 or C, treated for 6 months with 5-fluorouracil plus leucovorin was retrieved. The G2677T and C3435T polymorphisms were studied and immunohistochemical analysis of the tumor and adjacent normal tissue was performed. Results: The distribution of wild-type and polymorphic genotypes was similar in the patients and controls and in the patients who relapsed and those who remained event-free for 5 years. Cox proportional hazard model indicated an increased probability of relapse for older patients (p=0.042) and C stage tumors (p=0.030). Pgp expression was significantly lower in cancer tissue compared to normal mucosa (p

Original languageEnglish
Pages (from-to)3921-3928
Number of pages8
JournalAnticancer Research
Volume28
Issue number6 B
Publication statusPublished - Nov 2008

Keywords

  • ABCB1 gene
  • Colon cancer
  • Immunohistochemistry
  • P-glycoprotein
  • Polymorphisms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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