Abstract
Background: P-glycoprotein (Pgp), encoded by the ATP-binding cassette B1 (ABCB1) gene, is an efflux transporter located on the luminal side of intestinal epithelial cells, which protects the gut from endogenous and exogenous toxins. The association of two ABCB1 polymorphisms with the occurrence of colon cancer and long-term prognosis was evaluated in a selected patient population. The expression of Pgp in neoplastic and normal intestinal mucosa was also studied. Patients and Methods: Archival material from 51 patients, in Dukes stage B2 or C, treated for 6 months with 5-fluorouracil plus leucovorin was retrieved. The G2677T and C3435T polymorphisms were studied and immunohistochemical analysis of the tumor and adjacent normal tissue was performed. Results: The distribution of wild-type and polymorphic genotypes was similar in the patients and controls and in the patients who relapsed and those who remained event-free for 5 years. Cox proportional hazard model indicated an increased probability of relapse for older patients (p=0.042) and C stage tumors (p=0.030). Pgp expression was significantly lower in cancer tissue compared to normal mucosa (p
| Original language | English |
|---|---|
| Pages (from-to) | 3921-3928 |
| Number of pages | 8 |
| Journal | Anticancer Research |
| Volume | 28 |
| Issue number | 6 B |
| Publication status | Published - Nov 2008 |
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Keywords
- ABCB1 gene
- Colon cancer
- Immunohistochemistry
- P-glycoprotein
- Polymorphisms
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
ABCB1 gene polymorphisms and expression of P-glycoprotein and long-term prognosis in colorectal cancer. / De Iudicibus, Sara; De Pellegrin, Alessandro; Stocco, Gabriele; Bartoli, Fiora; Bussani, Rossana; Decorti, Giuliana.
In: Anticancer Research, Vol. 28, No. 6 B, 11.2008, p. 3921-3928.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - ABCB1 gene polymorphisms and expression of P-glycoprotein and long-term prognosis in colorectal cancer
AU - De Iudicibus, Sara
AU - De Pellegrin, Alessandro
AU - Stocco, Gabriele
AU - Bartoli, Fiora
AU - Bussani, Rossana
AU - Decorti, Giuliana
PY - 2008/11
Y1 - 2008/11
N2 - Background: P-glycoprotein (Pgp), encoded by the ATP-binding cassette B1 (ABCB1) gene, is an efflux transporter located on the luminal side of intestinal epithelial cells, which protects the gut from endogenous and exogenous toxins. The association of two ABCB1 polymorphisms with the occurrence of colon cancer and long-term prognosis was evaluated in a selected patient population. The expression of Pgp in neoplastic and normal intestinal mucosa was also studied. Patients and Methods: Archival material from 51 patients, in Dukes stage B2 or C, treated for 6 months with 5-fluorouracil plus leucovorin was retrieved. The G2677T and C3435T polymorphisms were studied and immunohistochemical analysis of the tumor and adjacent normal tissue was performed. Results: The distribution of wild-type and polymorphic genotypes was similar in the patients and controls and in the patients who relapsed and those who remained event-free for 5 years. Cox proportional hazard model indicated an increased probability of relapse for older patients (p=0.042) and C stage tumors (p=0.030). Pgp expression was significantly lower in cancer tissue compared to normal mucosa (p
AB - Background: P-glycoprotein (Pgp), encoded by the ATP-binding cassette B1 (ABCB1) gene, is an efflux transporter located on the luminal side of intestinal epithelial cells, which protects the gut from endogenous and exogenous toxins. The association of two ABCB1 polymorphisms with the occurrence of colon cancer and long-term prognosis was evaluated in a selected patient population. The expression of Pgp in neoplastic and normal intestinal mucosa was also studied. Patients and Methods: Archival material from 51 patients, in Dukes stage B2 or C, treated for 6 months with 5-fluorouracil plus leucovorin was retrieved. The G2677T and C3435T polymorphisms were studied and immunohistochemical analysis of the tumor and adjacent normal tissue was performed. Results: The distribution of wild-type and polymorphic genotypes was similar in the patients and controls and in the patients who relapsed and those who remained event-free for 5 years. Cox proportional hazard model indicated an increased probability of relapse for older patients (p=0.042) and C stage tumors (p=0.030). Pgp expression was significantly lower in cancer tissue compared to normal mucosa (p
KW - ABCB1 gene
KW - Colon cancer
KW - Immunohistochemistry
KW - P-glycoprotein
KW - Polymorphisms
UR - http://www.scopus.com/inward/record.url?scp=58149160026&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=58149160026&partnerID=8YFLogxK
M3 - Article
C2 - 19192650
AN - SCOPUS:58149160026
VL - 28
SP - 3921
EP - 3928
JO - Anticancer Research
JF - Anticancer Research
SN - 0250-7005
IS - 6 B
ER -