Ablation of Perk in schwann cells improves myelination in the S63del charcot-marie-tooth 1B mouse

Mariapaola Sidoli, Nicolò Musner, Nicholas Silvestri, Daniela Ungaro, Maurizio D’Antonio, Douglas R. Cavener, M. Laura Feltri, Lawrence Wrabetz

Research output: Contribution to journalArticle

Abstract

In factory cells, the accumulation of misfolded protein provokes the unfolded protein response (UPR). For example, deletion of serine 63 (S63del) in myelin protein zero (P0) induces P0 accumulation in the endoplasmic reticulum (ER) of Schwann cells and a persistent UPR associated with Charcot-Marie-Tooth 1B (CMT1B) demyelinating peripheral neuropathy in human and mouse. PERK (protein kinase RNA-like ER kinase) is the ER stress sensor that attenuates global translation by phosphorylating eIF2α. Inhibition of the eIF2α holophosphatase GADD34: PP1, increases the phosphorylation of eIF2α in Schwann cells and largely rescues S63del neuropathy. Nonetheless, reducing phosphorylation of eIF2α, by Perk haploinsufficiency, also ameliorates the myelin defects of S63del nerves. This contradictory finding prompted us to investigate whether the beneficial effect of Perk deficiency on myelination could derive from neurons. To test this hypothesis, we generated and compared Schwann cell-and neuron-specific ablation of Perk in S63del nerves. Our data suggest that the detrimental effect of Perk in CMT1B derives primarily from Schwann cells. Furthermore, we show that Perk loss of function in Schwann cells restores myelination without diminishing accumulation of P0 or markers of ER stress, suggesting that Perk may modulate myelination through a pathway independent of the UPR.

Original languageEnglish
Pages (from-to)11350-11361
Number of pages12
JournalJournal of Neuroscience
Volume36
Issue number44
DOIs
Publication statusPublished - Nov 2 2016

Keywords

  • Charcot-Marie-Tooth
  • Myelin
  • PERK
  • Proteostasis
  • Schwann cells
  • Unfolded protein response

ASJC Scopus subject areas

  • Neuroscience(all)

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  • Cite this

    Sidoli, M., Musner, N., Silvestri, N., Ungaro, D., D’Antonio, M., Cavener, D. R., Laura Feltri, M., & Wrabetz, L. (2016). Ablation of Perk in schwann cells improves myelination in the S63del charcot-marie-tooth 1B mouse. Journal of Neuroscience, 36(44), 11350-11361. https://doi.org/10.1523/JNEUROSCI.1637-16.2016